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Product Category: | Knockdown Validated Polyclonal Antibody |
Synonyms: | Actin β; β-Actin; Actin Beta; Actin, Cytoplasmic 1; PS1TP5-Binding Protein 1; Beta Cytoskeletal Actin; I (2)-Actin; Beta-Actin; PS1TP5BP1; B-Actin; BRWS1 |
UniProt Entry: | P60709 |
NCBI Gene Entry: | 60 |
Molecular Weight: | 45 kDa |
Clonality: | Rabbit polyclonal antibody |
Species Reactivity: | Human |
Applications: | WB/IC/F |
Recommended Dilution: | WB 1:1000 / IC 1:100 / F 1:100 |
Immunogen: | Recombinant fragment of human ACTB |
Isotype: | Rabbit IgG |
Storage Buffer: | Supplied in PBS (pH 7.5) containing 50 µg/ml BSA, 50% glycerol, and 0.01% sodium azide |
Storage: | At –20°C |
Description: | Beta actin, also named as ACTB and F-Actin, belongs to the actin family. Actins are highly conserved globular proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. At least six isoforms of actins are known in mammals and other vertebrates: alpha (ACTC1, cardiac muscle 1), alpha 1 (ACTA1, skeletal muscle) and 2 (ACTA2, aortic smooth muscle), beta (ACTB), gamma 1 (ACTG1) and 2 (ACTG2, enteric smooth muscle). Beta and gamma 1 are two non-muscle actin proteins. Most actins consist of 376aa, while ACTG2 (rich in muscles) has 375aa and ACTG1(found in non-muscle cells) has only 374aa. Beta actin has been widely used as the internal control in RT-PCR and Western Blotting as a 42-kDa protein. However, the 41 kDa cleaved fragment of beta actin can be generated during apoptosis process. This antibody can recognize all the actins. |
Documents: | Please contact us to request the manual |