Anti-BrdU Mouse mAb (100 μl)

Product Usage Information

Background

Bromodeoxyuridine (5-bromo-2'-deoxyuridine, BrdU, BUdR, BrdUrd) is a synthetic nucleoside that is an analog of thymidine. It can be incorporated into the newly synthesized DNA of replicating cells (during the S phase of the cell cycle), substituting for thymidine during DNA replication. As such, BrdU is used for birth dating and monitoring cell proliferation. BrdU is a toxic and mutagenic substance. It triggers cell death, the formation of teratomas, alters DNA stability, lengthens the cell cycle, and has mitogenic, transcriptional and translational effects on cells that incorporate it.

Images

	IHC analysis of Brdu (GB12051). Sample: Mouse jejunum (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: 2M HCL in pure water，37°C, 30minBlocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 500, 4°C overnight. Secondary antibody: HRP Goat Anti-Mouse lgG (GB23301), 1: 200 RT 1h.
	IHC analysis of Brdu (GB12051). Sample: Mouse jejunum (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: 2M HCL in pure water，37°C, 30minBlocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 500, 4°C overnight. Secondary antibody: HRP Goat Anti-Mouse lgG (GB23301), 1: 200 RT 1h.
	IF analysis of Brdu (GB12051). Sample: Mouse neonatal mouse's brain (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: TE buffer (pH 9.0) (G1203),98°C,20min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 500, 4°C overnight. Secondary antibody: Cy3 conjugated Goat Anti-mouse IgG (H+L)(GB21301),1: 200 RT 1h.

Storage

Publications

Li, M., et al. A ROS-responsive hydrogel incorporated with dental follicle stem cell-derived small extracellular vesicles promotes dental pulp repair by ameliorating oxidative stress. Bioact Mater 36,524-540(2024). PMID 39072284, IF 18.9

Zhong, T., et al. A wireless battery-free eye modulation patch for high myopia therapy. Nat Commun 15,1766(2024). PMID 38409083, IF 14.7

Huang, B., et al. Near-infrared-IIb emitting single-atom catalyst for imaging-guided therapy of blood-brain barrier breakdown after traumatic brain injury. Nature Communications 14,197(2023). PMID 36639379, IF 16.6

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