Primary Hepatic Stem Cells

HpSCs are essential for the normal function of liver. HpSCs are not only critical for researches such as the function of liver, interaction of different types of cells in liver and mechanism of regeneration of liver after injury, but also for researches like tumor suppression and Immuno-regulation. Importantly, HpSCs have the potential therapeutic application in acute liver injury, obesity related liver damage, nonalcoholic steatohepatitis (NASH), steatohepatitis (ASH), liver fibrosis, liver cirrhosis and hepatocellular carcinoma (HCC).

The human HpSCs (hHpSCs, Right Figure) have guaranteed highest quality, full function to differentiate to hepatocytes and bile duct epithelial cells, are reliable hHpSCs source for your research.

Publications

Human liver stem cells attenuate concanavalin A-induced acute liver injury by modulating myeloid-derived suppressor cells and CD4+ T cells in mice. Stem Cell Research & Therapy, 201910:22

Protective effect of intraperitoneal transplantation of human liver-derived stem cells at different times against concanavalin A-induced acute liver injury in mice. 2017 Mar 20;25(3):205-210

Case Study

1. Expressing hepatic stem cell specific markers

Figure 1: Markers. A) Western Blot of Albumin (ALB) and CK19. Control is hHpSC sample who expresses liver stem cell specific markers albumin and CK19. After induced to liver cell by 2% DMSO + HGF, only albumin can be detected by not CK19. B) hHpSCs express markers of both biliary epithelial cells (CK7, CK19) and hepatocytes lineages (ALB, CK8).

2. Positive results of indigo test and glycogen staining of hHpSCs

Figure 2: The hHpSCs shows positive in Indigo test indicating that the hHpSCs has normal liver function (green); Periodic acid-Schiff (PAS) is commonly used special stain in quantities of glycogen, which are mainly stored in the liver. PAS staining shows positive indicating that the hHpSCs has normal liver function (pink).

3. Anti-ConA induced acute liver injury function

Figure 3: Anti-ConA Induced acute liver injury by hHpSC. Eight mice in each group. C group: normal mice as control; M group: ConA treated; E group: hHpSC+ConA. At certain time point after intraperitoneal injection of hHpSC + ConA was injected through introvenous; then Alanine aminotransferase Test (ALT) was measured.

4. Protection of liver damage caused by CCl4

Figure 4: Protection of liver damage caused by CCl4. Liver fibrosis revealed by Sirius red staining for collagens. Top row: Representative images showing the development of advanced fibrosis induced by CCl4, which includes formation of nodules (cirrhosis) and linking septa without apparent nodular formation (bridging fibrosis). Bottom row: Representative images showing fibrosis in mice treated with CCl4 and were also given hHpSCs. Short septa and focal fibrotic tissue are visible.

5. Up-regulation of albumin (ALB) expression after inducing by DMSO.

Figure 5: qRT-PCR results of expression of ALB. ALB is commonly made by liver, ALB level was more than double after 15 days inducing by DMSO.

6. Prevention of ethanol-induced fatty liver by hHpSCs

Figure 6: The ethanol-induced fatty liver model, 7- to 8-week old male C57BL/6NCR mice were fed an ethanol-containing liquid Lieber-De- Carli diet or control diet whereby ethanol was substituted isocalorically with dextrin maltose for 6 weeks. For cell transplantation, the ethanol-fed mice, were given IP either phosphate buffer saline (PBS) or 2 × 106 hHpSCs , twice a week for 6 weeks. After 6 weeks of feeding, the mice developed significant steatosis of the liver and hHpSCs treatment ameliorates fatty livers.

Product Name	Cat. #	Size	Price	Order
Human Primary Hepatic Stem Cells	C0011	≥1×106/Vial	$1,299
Mouse Primary Hepatic Stem Cells	C0012	≥1×106/Vial	$899
Rat Primary Hepatic Stem Cells	C0013	≥1×106/Vial	$899

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