Anti-Flag Tag Mouse mAb (100 μl)

Anti-Flag Tag Mouse mAb (100 μl)

Brand: ServiceBio
Cat. #: GB12938-100
Availability: In Stock
$259.00
-+
Applications: WB    
Host Species: Mouse    
Clonality: Monoclonal    
Gene Name: Flag Tag
Synonyms: DDDDK tag, DYKDDDDK, ddk, DDDDK epitope tag, ECS epitope tag, Enterokinase Cleavage Site tag, FLAG tag, FLAG
Immunogen:

KLH conjugated Synthetic peptide corresponding to DYKDDDDK

Isotype: IgG2b,κ
Purity: Affinity purification
Documents: Manual-Flag tag antibody




Product Usage Information

WB

Species independent

1: 500-1: 1000

transfected 293T cells




Background

The DYKDDDDK(FLAG) peptide has been used extensively as a general tag in expression vectors. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion. N-terminal DDDDK vectors provide an Ek cleavage site for removal of the fusion tag. The DDDDK peptide is likely to be located on the surface of a fusion protein because of its hydrophilic nature. As a result, the DDDDK peptide is more likely to be accessible to antibodies. A DDDDK-tag can be used in many different assays that require recognition by an antibody, such as western blotting, immunocytochemistry, immunoprecipitation, flow cytometry, protein purification, and in the study of protein-protein interactions, cell ultrastructure, and protein localization and so on.




Images

flag tag antibodyWestern blot analysis of Flag-Tag (GB12938) at dilution of 1: 1000




Storage

StorageStore at -20°C for one year. Avoid repeated freeze/ thaw cycles.
Storage BufferPBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol.


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