Your shopping cart is empty!
| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, FC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | cofilin 1 |
| Gene Symbol: | CFL1 |
| Synonyms: | CFL; cofilin; HEL-S-15 |
| Gene ID: | 1072 |
| UniProt ID: | P23528 |
| Clone ID: | 24GB4775 |
| Immunogen: | A synthesized peptide derived from human Cofilin |
| Dilution: | WB 1:1,000-1:5,000; FC 1:2,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
The protein CFL1 encoded by this gene can polymerize and depolymerize F-actin and G-actin in a pH-dependent manner. Increased phosphorylation of this protein by LIM kinase aids in Rho-induced reorganization of the actin cytoskeleton. Cofilin is a widely distributed intracellular actin-modulating protein that binds and depolymerizes filamentous F-actin and inhibits the polymerization of monomeric G-actin in a pH-dependent manner. It is involved in the translocation of actin-cofilin complex from cytoplasm to nucleus.
Images
 | Western blotting analysis using anti-cofilin 1 antibody (Cat#63264). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-cofilin 1 antibody (Cat#63264, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Western blotting analysis using anti-cofilin 1 antibody (Cat#63264). Cofilin 1 expression in wild type (WT) and cofilin 1 (CFL1) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-cofilin 1 antibody (Cat#63264, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Flow cytometric analysis of cofilin 1 expression in H9c2 cells using anti-cofilin 1 antibody (Cat#63264, 1:2,000). Green, isotype control; red, cofilin 1. |
You may also be interested in:
