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| Key Feature: | • The PCR products contain A at the 3'-end and can be directly cloned into T-Vectors. • The extension rate is about 100b/sec. • Amplification of genomic DNA fragment up to 4 kb. • Very low cost and ideal for genotyping. |
| Description: | LiTaqTM DNA Polymerase is a thermostable DNA polymerase that exhibits a 5' → 3' polymerase activity and a 5' →3' exonuclease activity, with no 3' → 5 ' exonuclease activity. LiTaqTM DNA Polymerase is purifed from an Escherichia coli (E.coli) strain overexpressing the gene of Thermus aquaticus DNA Polymerase. No endonuclease, exonuclease, or bacterial DNA were detected in this product. The PCR products contain A at the 3'-end and can be directly cloned into T-Vectors. The products are also compatible with LiCloneTM One Step DNA Assembly Kit (Cat. #: M0010). |
| Applications: | • Routine PCR • Colony PCR |
| Unit Definition: | One unit (U) is defined as the amount of enzyme that incorporates 10 nmol of dNTPs into acid-insoluble products in 30 minutes at 74°C, with activated salmon sperm DNA as the template / primer. |
| Quality Control: | Exonuclease Activity: The product is tested in a reaction containing 10 U of Taq DNA Polymerase and 0.6 µg of λ-Hind III. After incubation at 37°C for 16 hours, there is no visually discernible change to DNA bands determined by agarose gel electrophoresis. Endonuclease Activity: The product is tested in a reaction containing 10 U of Taq DNA Polymerase and 0.6 µg of Supercoiled pBR322. After incubation at 37°C for 4 hours, there is no visually discernible change to DNA band determined by agarose gel electrophoresis. Functional Assay: The human α-1-antitrypsin gene is amplified for 30 cycles in a 50 µl system using 1.25 U of Taq DNA Polymerase and 100 ng human genomic DNA as template. A single DNA band of 360 bp is detected by 1% agarose gel electrophoresis. |
| Component: | 1. 10× Taq Buffer (Mg2+ plus): 4 ml 2. LiTaqTM DNA Polymerase (5 U/µl): 200 µl |
| Storage: | At -20°C for two years |
| Documents: | Manual |