LiScript™ One Step RT-PCR Kit is specially designed for RNA detection (such as RNA virus). With the One Step RT-PCR Kit and gene-specific primers (GSP), both reverse transcription and PCR amplification are performed in the same tube, with no additional pipetting procedures, which improves detection through-put and minimizes potential contamination. This kit contains Reverse Transcriptase, Taq plus hot-start DNA Polymerase, and an optimized buffer, which enables high-sensitive total RNA detection and long-fragment amplification (as long as 10 kb).
• Ready-to-use master mix: the reverse transcription and PCR are in the same tube
• Super sensitivity: able to detect total RNA as little as 0.1 pg
• Long-fragment amplification: able to amplify long fragments as long as 10 kb
A. Taking total RNA of Hela cells as template, the GAPDH fragment of 555 bp was amplified using LiScript™ One Step RT-PCR Kit. The detection sensitivity can reach 0.1 pg.
B. Taking PPRSV RNA as template, a fragment of 660 bp was amplified using LiScript™ One Step RT-PCR Kit. The detection sensitivity can reach 0.2 TCLD50.
C. Taking 1 μg of total RNA from Hela cells as template, fragments with different lengths were amplified using LiScript™ One Step RT-PCR Kit. The UTRN fragments as long as 12.1 kb were successfully amplified.
Product Name | Cat. # | Size | Price | Order |
LiScript™ One Step RT-PCR Kit | M0022 | 100 rxn | $299 |
Product Name | Cat. # | Size | Price | |
LiDirect™ Lightning Genotyping Kit | M0015 | 400 rxn | $139 | Read more |
2× LiTaq™ PCR Master Mix | M0024 | 15 ml | $189 | Read more |
2× LiSpark™ Ultra SuFi PCR Master Mix | M0031 | 1 ml | $129 | Read more |
LiQuant™ Green qPCR Master Mix | M0026 | 500 rxn | $199 | Read more |
LiQuant™ NGS dsDNA Assay Kit | M0066 | 500 rxn | $349 | Read more |
LiScript™ 1st Strand cDNA Synthesis Kit (+gDNA wiper) | M0028 | 50 rxn | $199 | Read more |
LiClone™ One Step DNA Assembly Kit | M0010 | 25 rxn | $199 | Read more |
LiClone™ Fast Mutagenesis Kit | M0011 | 20 rxn | $189 | Read more |
LiPure™ Endotoxin-Free Plasmid Mini Kit | M0017 | 50 rxn | $99 | Read more |
LiPure™ Gel Extraction Kit | M0035 | 200 rxn | $199 | Read more |
LiGreen™ DNA Gel Stain | M0049 | 1 ml | $149 | Read more |
dNTP Set (100 mM each) | M0059 | 4×250 µl | $139 $99 | Read more |