Virus Packaging Services

Virus Packaging Services

Adenovirus Packaging Service

Features:
1. Unsurpassable transduction efficiency (nearly 100% vs. AAV 30~40% and Lentivirus ~30%) in most mammalian cells.
2. No integration into host genome.
3. Compatible with toxic gene of interest (GOI).
4. Induce high-level transient protein expression.
5. Accommodate inserts of up to 7.5 kb.

Description:
1. Clone your gene of interest (GOI) into a shuttle vector followed by transfer of the whole transgene cassette to adenoviral genome.
2. Obtain initial adenoviral stock.
3. Adenovirus amplification from ~1400 cm2 Ad.MAX™ 293 cells.
4. Recombinant adenovirus PFU titration.

Turnaround time: 3~4 weeks

Required material: A NCBI accession # or gene symbol or plasmid DNA carrying your gene of interest (GOI).

rAAV Packaging Service

Features:
1. Nonpathogenic with least immune response.
2. Excellent gene delivery efficiency in most cell types including dividing and non-dividing or primary cells.
3. Persistent transgene expression.
4. Multiple serotypes (AAV-1, AAV-2, AAV-3, AAV-4, AAV-5, AAV-6, AAV-7, AAV-8, AAV-9, AAV-DJ/8 and AAV-DJ).

Description:
1. rAAV packaging in 10x150 mm plate.
2. rAAV purification via advanced 2xCsCl ultra-centrifugation.
3. Desalting, filter sterilization and AAV titration via qPCR.

Turnaround time: 1~2 weeks

Required material: AAV cis plasmid carrying your gene of interest (GOI), >100 µg.

Lentivirus Packaging Service

Features:
1. Derived from 3rd generation packaging system with a shRNA included for maximum yield.
2. An inhibitory trans element upstream of 5'-LTR allows packaging toxic transgenes.
3. LentiMAX™ packaging cell line is optimized for high pseudoviral titers.
4. Compatible with both 2nd and 3rd generation lentivectors.

Description:
1.  Lentivirus packaging via Lenti.MAX™ packaging system.
2. Lentivirus purification via PEG precipitation followed by ultra-centrifugation.
3. Recombinant lentivirus titration via p24 ELISA.

Turnaround time: 1~2 weeks

Required material: Lentivector (>50 µg) carrying your gene of interests (GOI) in transfection grade.

How to order?

1. Download Virus Packaging Quotation Form
2. Fill out the form and send email to sales@lifesct.com
3. Technical support will send you a quatation for your project in 1 business day
4. Call 1-240-715-2985 to place the order

                                                 

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