Human RUNX2 (Runt-Related Transcription Factor 2) ELISA Kit

Human RUNX2 (Runt-Related Transcription Factor 2) ELISA Kit

Cat. #: E-EL-H0925
Availability: 3-5 Business Days
$609.00
-+
Product Name:Human RUNX2 ELISA Kit    
Synonym:  
AML3; CBFA1; CCD; CCD1; OSF2; PEA2aA; PEBP2A1; PEBP2A2; PEBP2aA; PEBP2aA1; Runt-Related Transcription Factor 2
Background:
This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to RUNX2. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for RUNX2 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain RUNX2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of RUNX2. You can calculate the concentration of RUNX2 in the samples by comparing the OD of the samples to the standard curve.
Signaling Pathways:
Species:
Human
Detection Range:
0.313~20ng/mL
Sensitivity:
0.188ng/mL

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