Mouse IL-9 (Interleukin 9) ELISA Kit (96T)

Mouse IL-9 (Interleukin 9) ELISA Kit (96T)

Cat. #: E-EL-M0724
Availability: 3-5 Business Days
$580.00
-+
Product Name:  Mouse IL-9(Interleukin 9) ELISA Kit   
Synonym:  
IL9, HP40, P40, TCGFIII, TCGF3, P40 T-cell and Mast Cell Growth Factor, P40 Cytokine, T-cell Growth Factor P40
Background:
This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse IL-9. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse IL-9 and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse IL-9, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by adding Stop Solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse IL-9. The concentration of Mouse IL-9 in samples can be calculated by comparing the OD of the samples to the standard curve.
Species:
Mouse
Assay time:
4.5h
Detection Method:
Colormetric
Detection Range:
15.63—1000 pg/mL
Sensitivity:
9.38 pg/mL
Sample Type:
Serum, plasma and other biological fluids

Specificity

This kit recognizes some recombinant and natural Mouse IL-9. No significant cross-reactivity or interference was observed.

Typical data

For convenience in result calculation, absorbance can be used as ordinate and standard concentrations as abscissa. The standard curve provided in the manual is only for reference, and experimenters should draw the standard curve based on data of themselves.

(pg/mL) O.D Average Corrected
1000 2.465
2.523
2.494 2.41
500 1.693
1.739
1.716 1.632
250 0.953
0.925
0.939 0.855
125 0.446
0.472
0.459 0.375
62.5 0.297
0.281
0.289 0.205
31.25 0.194
0.178
0.186 0.102
15.63 0.127
0.145
0.136 0.052
0 0.074
0.094
0.084 --

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high leve Mouse IL-9 were tested 20 times on one plate, respectively. 
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mouse IL-9 were tested on 3 different plates, 20 replicates in each plate.

Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean
(pg/mL)
53.50 153.00 484.20 56.70 155.40 486.30
Standard
deviation
3.60 7.00 22.30 3.00 7.10 16.50
C V (%) 6.73 4.58 4.61 5.29 4.57 3.39

Recovery

The recovery of Mouse IL-9 spiked to three different levels in samples throughout the range of the assay in various matrices was evaluated.

Sample Type Range (%) Average Recovery (%)
Serum (n=5) 89-104 95
EDTA plasma (n=5) 90-102 97
Cell culture media (n=5) 83-97 90

Linearity

Samples were spiked with high concentrations of Mouse IL-9 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Serum (n=5) EDTA plasma (n=5) Cell culture media (n=5)
1:2 Range (%) 93-104 86-98 96-114
Average (%) 98 91 104
1:4 Range (%) 98-113 82-96 92-107
Average (%) 106 89 99
1:8 Range (%) 95-108 88-99 93-105
Average (%) 102 93 98
1:16 Range (%) 98-112 86-100 97-112
Average (%) 106 93 103

Kit components & Storage

The unopened kit can be stored at 4℃ for 1 month. If the kit is not used within 1 month, store the items separately according to the following conditions since the kit is received.

Item Specifications Storage
Micro ELISA Plate(Dismountable) 8 wells ×12 strips -20℃, 6 months
Reference Standard 2 vials
Concentrated Biotinylated Detection Ab (100×) 1 vial, 120 μL
Concentrated HRP Conjugate (100×) 1 vial, 120 μL -20℃(shading light), 6 months
Reference Standard & Sample Diluent IL-9

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