Rat INS(Insulin) ELISA Kit (96T)

Rat INS(Insulin) ELISA Kit (96T)

Cat. #: E-EL-R3034
Availability: 3-5 Business Days
$609.00
-+
Detection Range: 3.13 - 200 ng/mL   
Sensitivity: 1.88 ng/mL   
Species:
Rat   
Synonym:  
IDDM2, ILPR, IRDN, MODY10   
Background:

This ELISA kit can be applied to the in vitro quantitative determination of Rat INS concentrations in serum, plasma and other biological fluids.

This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat INS. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat INS and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat INS, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by adding Stop Solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat INS. The concentration of Rat INS in samples can be calculated by comparing the OD of the samples to the standard curve.

Sample Volume Required Per Well:
100 μL   
Sample Type:
Serum, plasma and other biological fluids   

Typical data

For convenience in result calculation, absorbance can be used as ordinate and standard concentrations as abscissa. The standard curve provided in the manual is only for reference, and experimenters should draw the standard curve based on data of themselves.

(ng/mL)O.DAverageCorrected
2002.253
2.299
2.2762.222
1001.449
1.479
1.4641.41
500.746
0.728
0.7370.683
250.392
0.4
0.3960.342
12.50.241
0.221
0.2310.177
6.250.149
0.131
0.140.086
3.130.097
0.103
0.10.046
00.047
0.061
0.054--

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high leve Rat INS were tested 20 times on one plate, respectively. 
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Rat INS were tested on 3 different plates, 20 replicates in each plate.

Intra-assay PrecisionInter-assay Precision
Sample123123
n202020202020
Mean
(ng/mL)
10.4024.1080.4010.9022.1074.30
Standard
deviation
0.601.203.100.701.002.90
C V (%)5.774.983.866.424.523.90

Recovery

The recovery of Rat INS spiked to three different levels in samples throughout the range of the assay in various matrices was evaluated.

Sample TypeRange (%)Average Recovery (%)
Serum (n=5)89-10395
EDTA plasma (n=5)88-10495
Cell culture media (n=5)92-107100

Linearity

Samples were spiked with high concentrations of Rat INS and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Serum (n=5)EDTA plasma (n=5)Cell culture media (n=5)
1:2Range (%)85-9894-10894-108
Average (%)91100100
1:4Range (%)100-11481-9196-109
Average (%)10686102
1:8Range (%)96-10886-9795-108
Average (%)10291102
1:16Range (%)94-10882-9397-108
Average (%)10188103

Kit components & Storage

The unopened kit can be stored at 4℃ for 1 month. If the kit is not used within 1 month, store the items separately according to the following conditions since the kit is received.

ItemSpecificationsStorage
Micro ELISA Plate(Dismountable)8 wells ×12 strips-20℃, 6 months
Reference Standard2 vials
INS

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