LiClone™ Fast MultiSite Mutagenesis Kit (25 rxn)

LiClone™ Fast MultiSite Mutagenesis Kit (25 rxn)

Cat. #: M0012
Availability: In Stock
Key Features: 

• LiSparkTM Max Super-Fidelity DNA Polymerase provides high-fidelity PCR with the lowest mutation rate.

• LiSparkTM Max Super-Fidelity DNA Polymerase with excellent long fragment amplification capability can be widely used for any plasmid amplification within 20 kb.

• Amplification is carried out exponentially and the template usage is extremely low, which is beneficial to the complete degradation of the original methylation template.

• DpnI eliminates contamination of the original template.

• The rapid LiCloneTM cloning system can efficiently cyclize PCR products.

• The amplified products can be directly used for recombinant reaction after digestion with DpnI.

• Mutations of three to five discontinuous sites (more than 50 bp apart) can be achieved in one reaction.

LiCloneTM Fast MultiSite Mutagenesis Kit is a site-directed mutagenesis system based on LiCloneTM rapid cloning technology. This kit can fulfill 3-5 separate site-directed mutagenesis in one reaction. This kit consists of two modules: LiSparkTM Max Super-Fidelity DNA Polymerase amplification module and LiCloneTM rapid cloning module. The ultra-fidelity of LiSparkTM Max Super-Fidelity DNA Polymerase significantly reduces the possibility of unexpected mutations during amplification of up to 20 kb in length. The LiCloneTM rapid cloning module allows homologous recombination reactions to replace the conventional annealing ring-forming reactions, requiring much less template and enables more flexible primer design strategy. The kit is equipped with Exnase MultiS, a recombinant enzyme specifically optimized for multi-sites-directed mutations. DpnI digestion of the specific amplification products can be directly applied in recombination reaction without purification. Highly optimized reaction buffer, fast operating procedures and amazing site-directed mutagenesis efficiency make LiCloneTM Fast MultiSite Mutagenesis Kit the preferred kit for DNA multi-sites mutation.
Applications: DNA site-directed mutagenesis
1. 2× Max buffer: 3×1.25 ml
2. dNTP Mix (10 mM each): 125 µl
3. LiSparkTM Super-Fidelity DNA Polymerase: 125 µl
4. DpnI (10 U/µl): 125 µl
5. 5× LC MultiS Buffer: 100 µl
6. Exnase MultiS: 50 µl
At -20°C for one year

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