• Simple, fast, and high efficient
• Homologous recombination with 1 - 5 fragments
• pCE-Zero vector is compatible with most PCR products
The LiClone cloning technology is simple, fast, and high efficient which enables directional insertion of any amplified DNA product into any linearized vector at any site. Firstly, the vector is linearized at the cloning site. A small sequence overlapped with each end of the cloning site is added onto the insert through PCR. The insert and the linearized vector, with overlapped sequences of 15 bp - 20 bp on both 5’- and 3’-end, respectively, are mixed in an appropriate ratio and incubated with recombinase Exnase at 50℃ for 5 - 15 min.
LiClone™ One Step DNA Assembly Kit is a novel cloning kit, applicable for homologous recombination with 1 - 5 fragments. The Kit is independent of DNA ligase, significantly reducing the self-ligated colonies. The enhanced Exnase and highly optimized buffer included in 2× LiClone Mix significantly improve the recombination efficiency and the tolerance to impurities. pCE-Zero vector is compatible with most PCR products, enabling the specific PCR products to be used directly for recombination without any treatments, which significantly simplify the procedure.
• Fast Cloning
• High-throughput Cloning
• Seamless Assembly
• Site-specific Mutagenesis
||1. 2× LiClone Mix: 125 µl
2. pCE-Zero Vector, Linearized (50 ng/ul): 25 µl
3. 500 bp Control Insert (20 ng/μl): 5 µl
||All components should be stored at -20°C; Avoid repeated freezing and thawing.
pCE-Zero Vector Map