|Key Features:||• The procedure is fast and simple, amenable to automation. The entire process takes less than 1 hour.
• Does not use hazardous chemicals such as phenol, chloroform, proteinase K or guanidine isothiocyanate.
• There is no need for mechanical disruption, organic extraction, column purification, or precipitation of the DNA.
• Cost-effective: just $0.30 per reaction.
||LiDirectTM Lightning Genotyping kit is a combination of LiDirectTM Genotyping DNA Extraction Buffer and LiTaqTM Plus PCR master mix. It is designed for fast extraction of tissue DNA and subsequent PCR analysis. LiDirectTM Genotyping kit contains all the reagents needed to rapidly (15 min) extract genomic DNA from mouse tails, yeast, plant cells and other tissues, cells, hair shafts, and saliva. It also include all reagents for subsequent genotyping PCR. The whole genotyping process can be completed within 1 hour.|
||1. LiDirectTM Extraction Buffer
2. LiDirectTM Stop Solution
3. 2× LiTaqTM Plus PCR Master Mix
||At -20°C for one year
||For the customers in DC, MD & PA, the shipping fee is free;
For the customers in other region of USA, the shipping fee is $20;
For the international customers, please contact us for inquiry.
||LiDirectTM Lightning Genotyping Kit (400 preps, $139)
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Taking advantage of our new proprietary formula, the LiDirectTM DNA extraction buffer enables fast DNA release from the toughest cells, and performs much better than traditional alkaline lysis protocols. Combined with our industry leading LiTaqTM Plus DNA polymerase, our LiDirectTM Lightning Genotyping kit helps scientists to genotype all types of tissues and cells effortlessly.
|Step 1: sample preparation
• 1~2 mm piece of mouse tail
• 5~10 mg of tissue
• ~1×104 cells
Step 2: add 75 µl of LiDirectTM Extraction Buffer
• Incubate at 98°C for 15 min
Step 3: add 125 µl of LiDirectTM Stop Solution
Step 4: set up 25 µl of LiTaqTM Plus PCR reaction mix
|PCR cycling for amplification:
1. 95°C: 15 sec
2. 35 ×
• Denaturation: 5 sec at 98°C
• Annealing: 10 sec at 55°C
• Extension: 30 sec/kb at 72°C
3. Final extension: 5 min at 72°C
From these results, it can be concluded that our LiDirectTM DNA extraction buffer is compatible with most Taq polymerases on the market, but our LiTaqTM plus product line is more robust than most other products on market.