LiPure™ Endotoxin-Free Plasmid Maxi Kit (10 rxn)

LiPure™ Endotoxin-Free Plasmid Maxi Kit (10 rxn)

Cat. #: M0019
Availability: In Stock
Key Features: 

• High yield: ~2.6 mg plasmid DNA from 100-300 ml of bacterial culture.

• High quality: the plasmid DNA can be used for transfection of most cell lines.

• Fast: the kit can be done in ~40 min.


LiPureTM Endotoxin-Free Plasmid Maxi Kit provides a simple, rapid andefficient method for the extraction of endotoxin-free plasmids.Based on conventional alkaline lysis method, the new and uniquesilicon membrane binds plasmid DNA efficiently and specifically.At the same time by using a special buffer system and endotoxinremoval filters, impurities such as endotoxin, genomic DNA, RNA,proteins are effectively removed.

100-300 ml of bacterial culture can be processed each time, andup to 2 mg of transfection-grade plasmid DNA can be obtained.The entire extraction process takes only 50 minutes. Theplasmids obtained from the kit have high purity and high yield andare particularly suitable for transfection experiments. Theplasmids can also be used for DNA sequencing, PCR, in vitrotranscription, restriction endonuclease digestion and otherexperiments.

1. Buffer P1: 125 ml
2. Buffer P2: 125 ml
3. Buffer E3: 125 ml
4. Buffer Ps: 30 ml
5. Buffer PW (concentrated): 50 ml
6. Endo-free Buffer EB: 30 ml
7. RNase A (10 mg/ml): 2 ml
8. Plungers: 10
9. Endo-Remover FQ: 10
10. Spin Columns DQ with Collection Tubes: 10
11. Centrifuge Tubes (50 ml): 10
Room temperature (15-30°C)

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