LiPureTM Endotoxin-Free Plasmid Midi Kit is specially designed to efficiently and rapidly extract plasmids from 15-50 ml bacterial cells. Based on conventional alkaline lysis method, the new and unique silicon membrane binds plasmid DNA efficiently and specifically, and each column can bind up to 250 ug plasmid DNA. Meanwhile by using a special buffer system and endotoxin-removal filters, impurities such as endotoxin, genomic DNA, RNA, proteins are effectively removed.
The plasmids obtained from the kit have high purity and high yield and are particularly suitable for transfection experiments. The plasmids can also be used for DNA sequencing, PCR, in vitro transcription, restriction endonuclease digestion and other experiments.
|Component:||1. Buffer P1: 30 ml|
2. Buffer P2: 30 ml
3. Buffer E3: 30 ml
4. Buffer PS: 15 ml
5. Buffer PW (concentrated): 10 ml
6. Endo-free Buffer EB: 30 ml
7. RNase A (10 mg/ml): 600 µl
8. Endo-Remover FX: 10
8. Plungers: 10
9. Spin Columns DX with Collection Tubes: 10
10. Centrifuge Tubes (15 ml): 10
|Storage:|| Room temperature (15-30°C)|