LiQuant™ Probe qPCR Master Mix (2500 rxns)

• The mix, prepared at 2× reaction concentration, can be directly used for robust and low-template quantitative PCR with high sensitivity, specificity and reliability.

• ROX reference dyes are provided for different qPCR instruments.

LiQuant™ Probe qPCR Master Mix is a special reagent designed for probe based qPCR. Utilizing hot-start LiQuant™ DNA polymerase, alone with optimal buffer specially optimized for qPCR, it is capable of significant restraining non-specific amplification as well as enhancing the amplification efficiency, thus is applicable for high-sensitivity qPCR reactions. It is a 2× premixed reagent which is convenient to handle. When using this product for qPCR reactions, reliable standard curve can be obtained in a wide range and accurate qualification of target genes can be achieved with high repeatability and reliability.

1. LiQuant™ Probe qPCR Master Mix:  25 ml

2. 50× ROX Reference Dye 1:  1000 μl

3. 50× ROX Reference Dye 2:  1000 μl

• Do Not Use ROX Reference Dye.

Bio-Rad CFX96™, CFX384™, iCycler iQ™, iQ™5, MyiQ™, MiniOpticon™, Opticon®, Opticon 2, Chromo4™; Cepheid SmartCycler®; Roche Applied Science LightCyclerTM 480r.

• Use ROX Reference Dye 1 (final 1×)

ABI 7900HT, 7300; StepOnePlus™

• Use ROX Reference Dye 2 (final 1×)

ABI 7500, 7500; Stratagene Mx3000P.