2× LiSpark™ Max SuFi PCR Master Mix (Free Sample)

2× LiSpark™ Max SuFi PCR Master Mix (Free Sample)

Cat. #: M0021-00
Availability: In Stock

For the customers from DC, MD, DE & PA, please contact us for free shipping.

$0.00
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Price in reward points: 200
Key Features: 

• Versatile: Used for routine PCR, or with long, difficult templates.

• Extreme Fidelity: 53× lower error rate than Taq DNA Polymerase.

• The extension rate is up to 200 bp/sec.

• Robust: Maximum success with minimal optimization, amplifies up to 40kb.

• High Yield: Increased product yield using minimal enzyme.

• No need to change buffer for GC-rich templates.

• More specific and higher yield than most polymerases on market.

Description:  
This product is a master mix of LiSparkTM Max Super-Fidelity DNA Polymerase, dNTP and optimized buffer system at 2× concentration. Therefore, amplification can be carried out as long as primers and templates are added, resulting in less frequent liquid relief operation and higher repeatability of flux and results. The protective agent in the system guarantees that LiSparkTM Max SuFi PCR Master Mix can keep its activity even after many freezing and thawing cycles. This product is capable of amplifying 20kb genomic DNA and 40kb λDNA, and will generate blunt ends compatible with LiClone™ One Step DNA Assembly Kit (M0010).

Applications: 

• High fidelity PCR

• High yield and fast PCR

• Blunt end cloning

• Complex templates

• GC-rich PCR

• Site-directed mutagenesis

Component:
1. 2× LiSparkTM Max SuFi PCR Master Mix
Storage:
At -20°C for one years
Shipping:
For the customers in DC, MD, DE & PA, the shipping fee is free;
For the customers in other region of USA, the shipping fee is $20;
For the international customers, please contact us for inquiry.
Other Size:
2× LiSparkTM Max SuFi PCR Master Mix (1 ml, $99)
2× LiSparkTM Max SuFi PCR Master Mix (5×1 ml, $299)
2× LiSparkTM Max SuFi PCR Master Mix (15×1 ml, $749)
Download:
Manual

Case Study

Samples: Human Genomic DNA & cDNA, Blood Samples, Mouse Tail Lysate

LifeSct: 2× LiSparkTM Max SuFi PCR Master Mix, 2× LiSparkTM Ultra SuFi PCR Master Mix

Competitors: Takara PrimeStar Max, Thermo Platinum Pfx

PCR reaction setup for amplification:
• 2× LiSpark Buffer: 25 µl
• dNTP Mix (10 mM each ): 25 µl
• F1: 2 µl
• R1 : 2 µl
• Human gDNA : 1 
µl
• ddH2O: 18 
µl

PCR cycling for amplification:
1. 95°C: 3 min
2. 35 x
    1. Denaturation: 15 s at 95°C
    2. Annealing: 15 s at 63°C
    3. Extension: 30 sec/kb at 72°C
3. Final extension: 5 min at 72°C


From the above results, it can be concluded that LiSparkTM is suitable for the amplification of a variety of difficult templates. Under high GC, low template concentration and high inhibitor conditions, LiSparkTM DNA polymerase invariantly performed better than other polymerases. Although direct amplification of lysates is common practice for shorter fragments, amplification of longer fragments is more consistent with LiSparkTM DNA polymerase.

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