LiSpark™ SuFi PCR Master Mixes

LiSparkTM Ultra-Fidelity DNA Polymerase is a superior enzyme in this category for robust PCR with extreme fidelity, with an error rate 108-fold lower than that of Taq polymerase. LiSparkTM Max Super-Fidelity DNA Polymerase possesses 5´→ 3´polymerase activity, 3´→5´exonuclease activity and will generate blunt-ended products. This product also capable of amplifying long fragments such as 20kb genomic DNA and 40kb λDNA. The recommended applications include high yield PCR, GC-rich PCR, site-directed mutagenesis and high throughput PCR, etc.

Key Features

• Versatile: Used for routine PCR, or with long, difficult templates.
• Extreme Fidelity: >100 lower error rate than Taq DNA Polymerase.
• The extension rate is up to 4-6 kb/min.
• Robust: Maximum success with minimal optimization, amplifies up to 40kb.
• No need to change buffer for GC-rich templates.
• More specific and higher yield than most polymerases on market.

PCR Mix	Fidelity	Extension Rate	3' end	Amplicon Size	Hot Start	Yield	Starting Price
LiTaqTM	1×	2 kb/min	A	≤15 kb	No	++	$0.22/rxn
LiTaqTM Eco	1×	1 kb/min	A	≤10 kb	No	+	$0.16/rxn
LiTaqTM Plus	6×	2 kb/min	A	≤10 kb	No	+++	$0.29/rxn
LiSparkTM Max	54×	4 kb/min	Blunt	≤20 kb	Yes	+++++	$1.25/rxn
LiSparkTM Ultra	108×	6 kb/min	Blunt	≤20 kb	Yes	+++++	$1.99/rxn

Case Study

Samples: Human Genomic DNA & cDNA, Blood Samples, Mouse Tail Lysate

LifeSct: 2× LiSparkTM Max SuFi PCR Master Mix, 2× LiSparkTM Ultra SuFi PCR Master Mix

Competitors: Takara PrimeStar Max, Thermo Platinum Pfx

PCR reaction setup for amplification:
• 2× LiSpark Buffer: 25 µl
• dNTP Mix (10 mM each ): 25 µl
• F1: 2 µl
• R1 : 2 µl
• Human gDNA : 1 µl
• ddH2O: 18 µl

PCR cycling for amplification:
1. 95°C: 3 min
2. 35 x
1. Denaturation: 15 s at 95°C
2. Annealing: 15 s at 63°C
3. Extension: 30 sec/kb at 72°C
3. Final extension: 5 min at 72°C

From the above results, it can be concluded that LiSparkTM is suitable for the amplification of a variety of difficult templates. Under high GC, low template concentration and high inhibitor conditions, LiSparkTM DNA polymerase invariantly performed better than other polymerases. Although direct amplification of lysates is common practice for shorter fragments, amplification of longer fragments is more consistent with LiSparkTM DNA polymerase.

Product Name	Cat. #	Size	Price	Order
LiSparkTM Max SuFi DNA Polymerase	M0020-01	100 U	$99
LiSparkTM Max SuFi DNA Polymerase	M0020-05	500 U	$389
LiSparkTM Max SuFi DNA Polymerase	M0020-10	1000 U	$699
2× LiSparkTM Max SuFi PCR Master Mix	M0021-01	1 ml	$99
2× LiSparkTM Max SuFi PCR Master Mix	M0021-05	5×1 ml	$299
2× LiSparkTM Max SuFi PCR Master Mix	M0021-15	15×1 ml	$749
2× LiSparkTM Ultra SuFi PCR Master Mix	M0031-01	1 ml	$129 $109
2× LiSparkTM Ultra SuFi PCR Master Mix	M0031-05	5×1 ml	$399 $349