LiSpark™ SuFi PCR Master Mix

LiSpark™ SuFi PCR Master Mix

LiSpark™ Ultra-Fidelity DNA Polymerase is a superior enzyme in this category for robust PCR with extreme fidelity, with an error rate 108-fold lower than that of Taq polymerase. LiSpark™ Ultra-Fidelity DNA Polymerase possesses 5´→ 3´polymerase activity, 3´→5´exonuclease activity and will generate blunt-ended products. This product also capable of amplifying long fragments such as 20kb genomic DNA and 40kb λDNA. The recommended applications include high yield PCR, GC-rich PCR, site-directed mutagenesis and high throughput PCR, etc.

Key Features

• Versatile: Used for routine PCR, or with long, difficult templates.
• Extreme Fidelity: >100 lower error rate than Taq DNA Polymerase.
• The extension rate is up to 4-6 kb/min.
• Robust: Maximum success with minimal optimization, amplifies up to 40kb.
• No need to change buffer for GC-rich templates.
• More specific and higher yield than most polymerases on market.

PCR MixFidelityExtension Rate3' endAmplicon SizeHot StartYieldStarting Price
LiTaq™2 kb/minA≤15 kbNo++$0.22/rxn
LiTaq™ Plus2 kb/minA≤10 kbNo+++$0.29/rxn
LiSpark™ Ultra108×6 kb/minBlunt≤20 kbYes+++++$1.99/rxn
Product NameCat. #FeaturesRequest
2× LiSpark™ Ultra SuFi PCR Master MixM0031Super fidelity, cost-effectiveFree Product

Case Study

Samples: Human Genomic DNA & cDNA, Blood Samples, Mouse Tail Lysate

LifeSct: 2× LiSpark™ Ultra SuFi PCR Master Mix

Competitors: Takara PrimeStar Max, Thermo Platinum Pfx

PCR reaction setup for amplification:
• 2× LiSpark Buffer: 25 µl
• dNTP Mix (10 mM each ): 25 µl
• F1: 2 µl
• R1 : 2 µl
• Human gDNA : 1 
• ddH2O: 18 

PCR cycling for amplification:
1. 95°C: 3 min
2. 35 x
    1. Denaturation: 15 s at 95°C
    2. Annealing: 15 s at 63°C
    3. Extension: 30 sec/kb at 72°C
3. Final extension: 5 min at 72°C

From the above results, it can be concluded that LiSpark™ is suitable for the amplification of a variety of difficult templates. Under high GC, low template concentration and high inhibitor conditions, LiSparkTM DNA polymerase invariantly performed better than other polymerases. Although direct amplification of lysates is common practice for shorter fragments, amplification of longer fragments is more consistent with LiSpark™ DNA polymerase.

Product Name
Cat. #
2× LiSpark™ Ultra SuFi PCR Master MixM0031-011 ml
2× LiSpark™ Ultra SuFi PCR Master MixM0031-055×1 ml
LiSpark™ Ultra SuFi DNA PolymeraseM0030-01100 U
LiSpark™ Ultra SuFi DNA PolymeraseM0030-05500 U$559

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