LiScript™ One Step RT-PCR Kit

LiScript™ One Step RT-PCR Kit

LiScript™ One Step RT-PCR Kit is specially designed for RNA detection (such as RNA virus). With the One Step RT-PCR Kit and gene-specific primers (GSP), both reverse transcription and PCR amplification are performed in the same tube, with no additional pipetting procedures, which improves detection through-put and minimizes potential contamination. This kit contains Reverse Transcriptase, Taq plus hot-start DNA Polymerase, and an optimized buffer, which enables high-sensitive total RNA detection and long-fragment amplification (as long as 10 kb).

Key Features

• Ready-to-use master mix: the reverse transcription and PCR are in the same tube
• Super sensitivity: able to detect total RNA as little as 0.1 pg
• Long-fragment amplification: able to amplify long fragments as long as 10 kb

Case Study


A. Taking total RNA of Hela cells as template, the GAPDH fragment of 555 bp was amplified using LiScript™ One Step RT-PCR Kit. The detection sensitivity can reach 0.1 pg.

B. Taking PPRSV RNA as template, a fragment of 660 bp was amplified using LiScript™ One Step RT-PCR Kit. The detection sensitivity can reach 0.2 TCLD50.

C. Taking 1 μg of total RNA from Hela cells as template, fragments with different lengths were amplified using LiScript™ One Step RT-PCR Kit. The UTRN fragments as long as 12.1 kb were successfully amplified.

Product Name
Cat. #
Size
PriceOrder
LiScript™ One Step RT-PCR KitM0022100 rxn$299

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