LiScript™ First-Strand cDNA Synthesis Kit

LiScript™ First-Strand cDNA Synthesis Kit contains all the components necessary for the 1st strand cDNA synthesis. The products are suitable for PCR and qPCR. The 2× RT Mix contains an optimized buffer and dNTPs. The Enzyme Mix contains the Reverse Transcriptase and the RNase inhibitor. The Oligo-(dT)23VN has a better affinity to Ploy A+ RNA than Oligo (dT)18. In addition, random hexamers and gene-specific primers (GSP) are also optional.

Key Features

• Based on high-effective Reverse Transcriptase: with high thermal stability ensuring the complex RNA secondary structure be denatured to obtain longer cDNA.
• A wide range of template input amount of total RNA (from 1 pg to 5 μg). Amplify cDNA fragments as long as 20 kb.
• Anchored Oligo(dT)23VN: further improves the specificity, efficiency and sensitivity of the first-strand cDNA synthesis.
• With different primer selection strategy, the first-strand cDNA products can be widely used for many applications, including cloning, hybridization, PCR amplification, and Real Time PCR.

Case Study

cDNA fragments as long as 20 kb was successfully obtained from RNA templates using LiScript™ 1st Strand cDNA Synthesis Kit (Left Figure). Reverse transcription was performed using 1 μg total RNA of HeLa cells or total RNA (for Nebulin) of skeletal muscle cells as templates and Oligo(dT)23VN as primers. PCR amplification was performed using 1 μl of cDNA (as template) and DNA Polymerase.

LiScript™ has higher reverse transcription efficiency for complex RNA templates (Right Figure). Using 1 μg of total RNA of Hela cells as template, reverse transcription was performed using LiScript™ and a similar product of T brand, respectively. The reaction time is 5 min. Using 1 μl of cDNA as template, the high-GC regions of FIB (6100 bp) and Pole (7117bp) were amplified using LiQuant™ Green qPCR Master Mix, respectively.

LiScript™ has a wide and sensitive linear reverse transcription range for qPCR. Reverse transcription was perform using 1 μg-1 pg total RNA of Hela cells as template and random hexamers/Oligo(dT)23VN as mixed primers. qPCR amplification of amplify ACTB gene and BR3 gene was performed using 1 μl of cDNA (as template) and LiQuant™ Green qPCR Master Mix, respectively.

Product Name	Cat. #	Size	Price	Order
LiScriptTM First-Strand cDNA Synthesis Kit	M0029	100 rxn	$239
LiScriptTM First-Strand cDNA Synthesis Kit (+gDNA wiper)	M0028-10	100 rxn	$349