LiScript™ First-Strand cDNA Synthesis Kit contains all the components necessary for the 1st strand cDNA synthesis. The products are suitable for PCR and qPCR. The 2× RT Mix contains an optimized buffer and dNTPs. The Enzyme Mix contains the Reverse Transcriptase and the RNase inhibitor. The Oligo-(dT)23VN has a better affinity to Ploy A+ RNA than Oligo (dT)18. In addition, random hexamers and gene-specific primers (GSP) are also optional.
• Based on high-effective Reverse Transcriptase: with high thermal stability ensuring the complex RNA secondary structure be denatured to obtain longer cDNA.
• A wide range of template input amount of total RNA (from 1 pg to 5 μg). Amplify cDNA fragments as long as 20 kb.
• Anchored Oligo(dT)23VN: further improves the specificity, efficiency and sensitivity of the first-strand cDNA synthesis.
• With different primer selection strategy, the first-strand cDNA products can be widely used for many applications, including cloning, hybridization, PCR amplification, and Real Time PCR.
cDNA fragments as long as 20 kb was successfully obtained from RNA templates using LiScript™ 1st Strand cDNA Synthesis Kit (Left Figure). Reverse transcription was performed using 1 μg total RNA of HeLa cells or total RNA (for Nebulin) of skeletal muscle cells as templates and Oligo(dT)23VN as primers. PCR amplification was performed using 1 μl of cDNA (as template) and DNA Polymerase.
LiScript™ has higher reverse transcription efficiency for complex RNA templates (Right Figure). Using 1 μg of total RNA of Hela cells as template, reverse transcription was performed using LiScript™ and a similar product of T brand, respectively. The reaction time is 5 min. Using 1 μl of cDNA as template, the high-GC regions of FIB (6100 bp) and Pole (7117bp) were amplified using LiQuant™ Green qPCR Master Mix, respectively.
LiScript™ has a wide and sensitive linear reverse transcription range for qPCR. Reverse transcription was perform using 1 μg-1 pg total RNA of Hela cells as template and random hexamers/Oligo(dT)23VN as mixed primers. qPCR amplification of amplify ACTB gene and BR3 gene was performed using 1 μl of cDNA (as template) and LiQuant™ Green qPCR Master Mix, respectively.
|Product Name||Cat. #||Size||Price||Order|
|LiScriptTM First-Strand cDNA Synthesis Kit||M0029||100 rxn||$239|
|LiScriptTM First-Strand cDNA Synthesis Kit (+gDNA wiper)||M0028-05||50 rxn||$199|
|LiScriptTM First-Strand cDNA Synthesis Kit (+gDNA wiper)||M0028-10||100 rxn||$349|
|Product Name||Cat. #||Size||Price|
|LiDirect™ Lightning Genotyping Kit||M0015||400 rxn||$139||Read more|
|2× LiTaq™ PCR Master Mix||M0024||15 ml||$189||Read more|
|2× LiSpark™ Ultra SuFi PCR Master Mix||M0031||1 ml||$129||Read more|
|LiQuant™ Green qPCR Master Mix||M0026||500 rxn||$199||Read more|
|LiQuant™ NGS dsDNA Assay Kit||M0066||500 rxn||$349||Read more|
|LiScript™ One Step RT-PCR Kit||M0034||50 rxn||$199||Read more|
|LiClone™ One Step DNA Assembly Kit||M0010||25 rxn||$199||Read more|
|LiClone™ Fast Mutagenesis Kit||M0011||20 rxn||$189||Read more|
|LiPure™ Endotoxin-Free Plasmid Mini Kit||M0017||50 rxn||$99||Read more|
|LiPure™ Gel Extraction Kit||M0035||200 rxn||$199||Read more|
|LiGreen™ DNA Gel Stain||M0049||1 ml||$149||Read more|
|dNTP Set (100 mM each)||M0059||4×250 µl||$139 $99||Read more|