PCNA Polyclonal Antibody (20 μl)  

Cat. #: 6151S
Availability: In Stock
Product Category:   Knockdown Validated Polyclonal Antibody
Synonyms:   Proliferating Cell Nuclear Antigen; Cyclin; DNA Polymerase Delta Auxiliary Protein; ATLD2
UniProt Entry:   P12004 
NCBI Gene Entry:   5111 
Molecular Weight:   36 kDa 
Clonality:   Rabbit polyclonal antibody
Species Reactivity:   Human
Applications:   WB/IC/F 
Recommended Dilution:   WB 1:1000 / IC 1:200 / F 1:100
Immunogen:   Recombinant fragment of human PCNA
Isotype:   Rabbit IgG 
Storage Buffer:   Supplied in PBS (pH 7.5) containing 50 µg/ml BSA, 50% glycerol, and 0.01% sodium azide  
Storage:   At –20°C
Background:    Proliferating Cell Nuclear Antigen,commonly known as PCNA,is a protein that acts as a processivity factor for DNA polymerase δ in eukaryotic cells. This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. PCNA induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase,but not apurinic-apyrimidinic (AP) endonuclease,APEX2 activities. It has to be loaded onto DNA in order to be able to stimulate APEX2. PCNA protein is highly conserved during evolution; the deduced amino acid sequences of rat and human differ by only 4 of 261 amino acids. PCNA has been used as loading control for proliferating cells.
Documents:   Request a manual/MSDS

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