PeneFect™ Transfection Reagent (1 ml)

PeneFect™ Transfection Reagent (1 ml)

Cat. #: M0001-01
Availability: In Stock
$149.00
-+
Key Features: 1. Bio-degradable after endocytosis    
2. Exceptional high titers of virus production
3. Equally good for very long DNAs (>89 kb)
4. Equally good for both single DNA transfection and multi DNA co-transfection
5. High levels of recombinant protein production
6. Simple & robust transfection procedure
7. Very affordable
Description: 
Based on our proprietary polymer synthesis technology, PeneFectTM Transfection Reagent is formulated as a biodegradable polymer based DNA transfection reagent that ensures effective and reproducible transfection on HEK293, COS-7, NIH-3T3, HeLa, CHO and a broad ranges of hard-to-transfect mammalian cells. PeneFectTM reagent is able to immobilize DNA migration during electrophoresis at very low concentration and form transfection complex within 5 minutes at RT. A remarkable feature of the reagent is the rapid and complete degradation of polymer after transfection complex endocytosis , leading to much less cytotoxicity. PeneFectTM reagent, 1.0 ml, is sufficient for ~667 transfections in 24 well plates or ~333 transfections in 6 well plates, providing a very affordable alternative to the leading products for transfecting a variety of commonly used and hard-to-transfect mammalian cells.
Storage Condition:
Store at 4 °C. If stored properly, the product is stable for 12 months or longer.
Download:
General Protocol
Protocol for Suspension 293 and CHO Cells
Protocol for Lentivirus Production
Protocol for rAAV Production

Broad Transfection Spectrum for Mammalian Cell Types

Cell Lines

Efficiency (% GFP)

Cell Lines

Efficiency (% GFP)

McArdle 7777
Hep3D
SHEP
3T3-442A
COS-7
CV-1
D 407
DHD Pro.b
3LL
B16-F10
BAEC
BHK-21
Ca Ski
CaCo2
CHO
HCS-2/8
HEK-293
HeLa
HLMEC
H-MVEC
Huh-7D12
ATT20
SK-N-SH
C2C12
HepG2

65-70%
67-76%
68-71%
35%
85-90%
60%
70%
70%
80%
85%
51%
80%
88%
60%
88%
61%
86%
88%
72%
59%
72%
46%
29%
46%
72%

hESCs
SN56

MC3T3-E1
Primary melanocyte
mESCs
L929
MCF-7
MDCK
Neuro2A
NIH 3T3
PC12
SH-SY5Y
SiHa
SKOV3
Huh-7
IGROV1
DF-1, Chicken Embryonic Cell
6CSFMEo
WEHI 231
A549
LNCap
Prim. mouse keratinocyte
Prim. human skin fibroblast
Prim. human pre-adipocyte
Prim. mouse embry. fibroblast

70%
81%
80%
35%
70%
59%
68%
68%
86%
76%
50%
25%
60%
65%
70%
35%
50%
71%
26%
75%
75
29%

50%
32%
30%


Examples Showing Transfection Efficiency of PeneFectTM In Vitro DNA Transfection Reagent on Commonly Used Cells


Transfection efficiency comparison of PeneFectTM vs. Lipofectamine Plus on Chinese Hamster Ovary (CHO) cells. HA tagged beta-tubulin cDNA was delivered into CHO cells with PeneFectTM (left panel) and lipofectamine Plus (right panel) respectively. FITC conjugated antibody against HA tag was utilized to pick up HA-beta-tubulin (Green) while a DM1a antibody was used to detect endogenous alpha-tubulin followed by probing with rhodamine conjugated secondary antibody (Red). 


A comparison showing transfection efficiency of PeneFectTM reagent vs. a leading product, Lipofectamine 2000 on HEK293FT cells. HEK-293T cells were transfected with GFP vector (pEGFP-N3) by PeneFectTM (left panel) and Lipofectamine 2000 (right panel) respectively. The cells were visualized by Nikon Eclipse Fluorescence microscope 24 hours post transfection


A comparison showing transfection efficiency of PeneFectTM reagent vs. a leading product, Lipofectamine 2000 on HepG2 cells. HepG2 cells were transfected with GFP vector (pEGFP-N3) by PeneFectTM (left panel) and Lipofectamine 2000 (right panel) respectively. The cells were visualized by Nikon Eclipse Fluorescence microscope 24 hours post transfection.


Transfection efficiency comparison of PeneFectTM vs. Fugene HD on MDCK cells. A plain GFP DNA was transduced into MDCK cells with PeneFectTM (left panel) and Fugene HD (right panel) reagents respectively per manufacturers' protocols. GFP and DAPI staining were visualized under fluorescence microscopy 48 hours post transfection. 


A comparison showing transfection efficiency of PeneFectTM reagent vs. a leading product, Lipofectamine 2000 on MDCK cells. MDCK cells are notoriously hard to transfect. With proprietary "Shaved Cell Transfection" protocol, PeneFectTM (left panel) gives up to 70% GFP positive cells vs. Lipofectamine 2000 (right panel) around 5% efficiency. MDCK cells were transfected with GFP vector (pEGFP-N3) by PeneFectTM (left panel) and Lipofectamine 2000 (right panel) respectively. The cells were visualized by Nikon Eclipse Fluorescence microscope 36 hours post transfection


A comparison showing transfection efficiency of PeneFectTM reagent vs. a leading product, Fugene HD on LNCap cells. LNCap cells were transfected with GFP vector (pEGFP-N3) by PeneFectTM (left panel) and Fugene HD (right panel) respectively. The cells were visualized by Nikon Eclipse Fluorescence microscope 24 hours post transfection


A image showing exceptional transfection efficiency of PeneFectTM reagent on Human embryonic stem cells (hESCs). The hESCs grown in E8 medium on Geltrexvs (left panel, DIC imaging) was transfected with pEF1α-GFP. The GFP expression (right panel) was visualized by Nikon Eclipse Fluorescence microscope 24 hours post transfection. 


Neuro2A cells transfected with pEGFP-C1 plasmid using PeneFectTM In Vitro DNA Transfection Reagent. The Neuro2A cells were visualized by Nikon Eclipse Fluorescence microscope with DIC phase imaging (left) and FITC imaging (right) 24 hours post-transfection


Comparison of cytotoxicity of PeneFectTM DNA In Vitro Transfection Reagent with L2K on primary murine skin fibroblast. The primary murine fibroblast was incubated with the indicated transfection reagents/pEGFP-C1 (DNA) complexes above for 4 hours in serum-free DMEM High Glucose medium followed by replacement of complete serum-containing medium. The cells were visualized by Nikon Eclipse Fluorescence microscope with DIC phase imaging 24 hours post transfection.

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