|Key Features:|| |
• Shorter time-consuming database construction.
• More efficient linker connection efficiency, higher library conversion rate.
• Wider template compatibility range, RNA starting amount can be as low as 10ng.
• Wide species applicability, can be applied to different species such as humans, animals, plants, etc.
VAHTS Universal V6 RNA-seq Library Prep Kit for Illumina is specially designed for the preparation of transcriptome libraries for next generationsequencing (NGS) platforms of Illumina®. The kit is universal and suitable for RNA library construction of RNA that have been obtained byPoly(A)-based mRNA enrichment or rRNA depletion . The kit contains two types of cDNA 2nd Strand synthesis buffer, which can be chosen for libraryconstruction for non-stranded transcriptome or stranded transcriptome analysis.
This kit combines 2nd Strand cDNA synthesis, end-repair and dA-Tailing into one step, with no need of purification, which greatly simplifies the processof library construction and shortens the operation time. The optimized reaction system improves the library construction efficiency, is compatible withlower-input RNA, and has uniform coverage for different amounts of input-RNA. Libraries of specific sizes, which can be customized, can be obtainedafter size selections with magnetic beads.
|Component:||1. Frag/Prime Buffer: 468 µl|
2. Actinomycin D (5mg/ml): 24 µl
3. 1st Strand Buffer 2: 144 µl
4. 1st Strand Enzyme Mix 2: 48 µl
5. 2nd Strand Buffer 2 (with dNTP): 600 µl
6. 2nd Strand Buffer 2 (with dUTP): 600 µl
7. 2nd Strand Enzyme Super Mix: 360 µl
8. Rapid Ligation Buffer 3: 600 µl
9. Rapid DNA Ligase 2: 120 µl
10. PCR Primer Mix 3: 120 µl
11. VAHTS HiFi Amplification Mix: 600 µl
12. Heat-labile UDG: 24 µl
|Storage:||All components should be stored at -20℃.|
LifeSct LLC is a high-tech biotech corporation, focusing on genomic and proteomic technology. LifeSct utilizes scientific excellence, a commitment to customer service and unsurpassed product quality to help our clients realize their research goals in the rapidly evolving world of genomics and proteomics.