| Applications: | WB, ICC/IF, IP |
| Host Species: | Mouse |
| Specificity: | His-Tag Mouse Monoclonal antibody detects endogenous levels of internal, C-terminal, or N-terminal 6*His -tagged proteins. |
| Immunogen: | A synthesized peptide |
| Dilution: | WB 1:1000-1:5000 ICC/IF 1:100-1:200 IP 1:100 |
| Purification Method: | Affinity-chromatography |
| Buffer: | 1 mg/ml in PBS, pH 7.4, containing 0.02% sodium azide and 50% glycerol. |
| Storage: | Store at -20°C and avoid freeze/thaw cycles. |
Images
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Western blot analysis of His-tagged protein expression in (1) 293T cell lysate; (2) 293T cell transfected with His-tagged protein lysate. |