DNA-Seq

DNA-Seq

Human whole genome sequencing enables researchers to catalog the genetic constitution of individuals and capture all the variants present in a single assay. It is applied to the study of cancer and a variety of diseases, as well as human population evolution studies and pharmacogenomics.
 

Key Features

1. State-of-the-art NGS technologies: LifeSct is a world leader in sequencing capacity using state-of-the-art technology, including the latest generation Illumina HiSeq X Ten.
2. Highest data quality: We guarantee a Q30 score ≥ 80%, exceeding Illumina’s official guarantee of ≥75%.
3. Extraordinary informatics expertise: LifeSct uses its cutting-edge bioinformatics pipeline and internationally recognized best-in-class software to provide customers highly reliable “publication-ready data”.
 

Online Quote

Please click the button on the right side and login to your account to request a quote. 

Service Options (Cat. #: S0026)

Project Description Unit Price Turnaround Time


Whole Genome Sequencing
(WGS) (HiSeq PE150, Q30≥80%)

Library Preparation $150/sample

 

15-20 business days

Sequencing $25/G
FTP $50


Service Overview

Sequencing Strategy: 1. 350 bp insert DNA library
2. HiSeq X platform, paired-end 150 bp
Data Quality Guarantee: We guarantee that ≥ 80% of bases have a sequencing quality score ≥ Q30, which exceeds Illumina’s official guarantee of ≥ 75%.
Sample Requirements: 1. Input DNA: For fresh sample: ≥ 1.0 μg (a minimum of 200 ng can be accepted with risk); For FFPE sample: ≥ 1.5 μg
2. DNA concentration: ≥ 20 ng/μl
3. DNA volume: ≥ 10 μl
4. Purity: OD260/280 = 1.8 - 2.0 without degradation or RNA contamination
Turnaround Time: 1. 15 working days after verification of sample quality (without data analysis)
2. Additional 8 working days for data analysis
Recommended Sequencing Depth: 1. For tumor tissues: 50×, adjacent normal tissues and blood 30×
2. For rare diseases: 30~50×
Bioinformatics Analysis: 1. Data quality control: filtering out reads containing adapters or with low quality
2. Alignment with reference genome, statistics of sequencing depth and coverage
3. SNP/InDel/SV/CNV calling, annotation and statistics
4. Somatic SNP/InDel/SV/CNV calling, annotation and statistics (paired tumor samples)

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