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Metagenomics Sequencing
| Background: | In metagenomics, genomes from environmental samples are analyzed without the prior isolation and cultivation of individual species, and, therefore, it is a powerful technique for studying microbial communities in their natural habitat, with a broad range of applications. |
| Features: | 1. Highly experienced: We have completed over 400 metagenomic sequencing projects for our customers, and have published multiple metagenomic studies. 2. Outstanding service: We provide high-quality sequencing, an efficient standard workflow, and bioinformatics analyses at a cost-effective price. 3. Effective methodology: Our techniques enhance the generation of data from low-abundance species. 4. Comprehensive analysis: Expert bioinformatics analysis with three databases (KEGG, eggNOG, CAZy) provides comprehensive data on annotated genes and metabolic pathways. |
| Sequencing Strategy: | 1. 300 bp insert DNA library 2. HiSeq platform, paired-end 150 bp |
| Data Quality Guarantee: | Each sample generates at least 5-10 Gb raw data, with Q30 ≥ 80%. |
| Sample Requirements: | 1. DNA amount: ≥ 0.8 μg (for one library preparation) 2. DNA concentration: ≥ 20 ng/μl 2. Purity: OD260/280 = 1.8 - 2.0 without degradation or RNA contamination |
| Turnaround Time: | 1. Within 15 business days from verification of sample quality (without data analysis) 2. Additional 10 business days for data analysis |
| Recommended Sequencing Depth: | Two strategies: 6 Gb raw data or 12 Gb raw data |
| Order Guide: | Please get a quote via sales@lifesct.com or secured online quotation |