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Labeled Nucleotides
LifeSct offers 5′-aminoallyl dUTP and UTP analogs, as well as conjugates with biotin and various fluorescent dyes, featuring our exceptionally bright and photostable LiFluor dyes. Fluorescent and biotin-labeled dUTP and UTP are widely used for enzymatic incorporation into DNA or RNA.
• Bright signals with low background
• Wide variety of fluorophores
• Great for many applications, like Fluorescence in situ Hybridization (FISH), TUNEL, and more
Fluorescence in situ Hybridization (FISH) of CEP7/MET probes labeled with LiFluor dyes. Human Chromosome 7 Enumeration (CEP7) and Human c-Met gene (MET) probes were labeled with LiFluor 488-X-dUTP (green) and LiFluor 568-X-dUTP (magenta), respectively, using nick translation. The labeled probes were mixed together in hybridization buffers with blocking DNA, and incubated with HT1080 cells on slides at 37°C overnight. The slides was washed and mounted with AntiFade solution containing DAPI (blue), then visualized with an Epi-Fluorescence microscope.
Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL)-mediated detection of apoptotic cells in mouse tongue tissue using Biotin-11-dUTP. Left: Cells were labeled with Biotin-11-dUTP, followed by detection with LiFluor™ 488-streptavidin (green). Nuclei were also counterstained with DAPI (blue). Right: Cells were labeled with Biotin-11-dUTP, followed by detection with horse radish peroxidase (HRP).
| Product Name | Cat. # | Size | Price | Order |
| 5-Aminoallyl-dUTP | M0070 | 100 μl | $149 |  |
| 5-Aminoallyl-UTP | M0071 | 100 μl | $149 | |
| Biotin-11-dUTP | M0072 | 50 μl | $199 | |
| Biotin-11-UTP | M0073 | 50 μl | $199 | |
| Biotin-14-dCTP | M0074 | 50 nmol | $369 | |
| 5-FAM-dUTP | M0075 | 25 μl | $169 | |
| 5-FAM-UTP | M0076 | 25 μl | $169 | |
| 5-FAM-X-UTP | M0077 | 25 μl | $199 | |
| 5-FAM-X-UTP | M0078 | 25 μl | $199 | |
| LiFluor™ 488-X-dUTP | M0079 | 25 μl | $299 | |
| LiFluor™ 488-X-UTP | M0080 | 25 μl | $299 | |
| LiFluor™ 555-X-dUTP | M0081 | 25 μl | $299 | |
| LiFluor™ 555-X-UTP | M0082 | 25 μl | $299 | |
| LiFluor™ 568-X-dUTP | M0083 | 25 μl | $299 | |
| LiFluor™ 568-X-UTP | M0084 | 25 μl | $299 | |
| LiFluor™ 594-X-dUTP | M0085 | 25 μl | $299 | |
| LiFluor™ 594-X-UTP | M0086 | 25 μl | $299 | |
| LiFluor™ 647-X-dUTP | M0087 | 25 μl | $299 | |
| LiFluor™ 647-X-UTP | M0088 | 25 μl | $299 | |
Related Products
| Product Name | Cat. # | Size | Price | |
| dNTP Set (100 mM each) | M0059 | 4×250 µl | $129 | Read more |
| CFSE Cell Proliferation Kit | M0004 | 1,000 rxn | $199 | Read more |
| Cell Viability Kit | C0027 | 1,000 rxn | $199 | Read more |
| CCK-8 Cell Counting Kit | C0024 | 500 rxn | $139 | Read more |
| TUNEL Chromogenic Apoptosis Detection Kit | C0036 | 50 rxn | $439 | Read more |
| LiFluor™ Fluorescent Dye | C0098 | 5 mg | $369 | Read more |
