LiScript™ One Step Green RT-qPCR Kit is designed for SYBR Green I-based qPCRs that directly use RNA as templates. The reverse transcription and PCR can be finished in one tube, significantly reducing pipetting procedures and the risk of contamination. The Reverse Trancriptase and Taq DNA Polymerase contained in this kit and an optimized buffer system enables high-sensitive total RNA detection (as little as 1 pg).
• Suitable for cost-effective and high-throughput gene expression analysis
• Unique ingredients to inhibit the formation of primer dimers, improving the quantification accuracy
• High amplification efficiency: able to detect total RNA as little as 1 pg
Taking total RNA from Hela cells as template, the B2M (A) and GUSB (B) gene were amplified using LiScript™ One Step Green qRT-PCR Kit and a similar kit of another brand, respectively. The kit demonstrates a higher amplification efficiency, indicated by a lower Ct value under the same template input and less primer dimers (almost none).
Product Name | Cat. # | Size | Price | Order |
LiScript™ One Step Green RT-qPCR Kit | M0032 | 100 rxn | $399 | |
LiScript™ One Step Probe RT-qPCR Kit | M0033 | 100 rxn | $399 |
Product Name | Cat. # | Size | Price | |
LiDirect™ Lightning Genotyping Kit | M0015 | 400 rxn | $139 | Read more |
2× LiTaq™ PCR Master Mix | M0024 | 15 ml | $189 | Read more |
2× LiSpark™ Ultra SuFi PCR Master Mix | M0031 | 1 ml | $129 | Read more |
LiQuant™ Green qPCR Master Mix | M0026 | 500 rxn | $199 | Read more |
LiQuant™ NGS dsDNA Assay Kit | M0066 | 500 rxn | $349 | Read more |
LiScript™ 1st Strand cDNA Synthesis Kit (+gDNA wiper) | M0028 | 50 rxn | $199 | Read more |
LiScript™ One Step RT-PCR Kit | M0034 | 50 rxn | $199 | Read more |
LiClone™ One Step DNA Assembly Kit | M0010 | 25 rxn | $199 | Read more |
LiClone™ Fast Mutagenesis Kit | M0011 | 20 rxn | $189 | Read more |
LiPure™ Endotoxin-Free Plasmid Mini Kit | M0017 | 50 rxn | $99 | Read more |
LiPure™ Gel Extraction Kit | M0035 | 200 rxn | $199 | Read more |
LiGreen™ DNA Gel Stain | M0049 | 1 ml | $149 | Read more |
dNTP Set (100 mM each) | M0059 | 4×250 µl | $139 $99 | Read more |