IntactProtein™ Cell-Tissue Lysis Kit (20 ml)

• All-in-one formula: No protease/PTM inhibitors needed; no sonication required.

• Ready-to-use protocol: Simply mix Reagents A&B; extraction in as little as 15 min.

• Ultimate solution for large proteins: Near-complete extraction; no fragmentation.

• Assurance and peace of mind: No loss of protein PTMs such as phosphorylation.

• All-around performance: Suitable for mammalian cells and tissues.

One of the key factors influencing Western blot results is the extraction of proteins from cells. In practice, detergent-based buffers such as radioimmunoprecipitation assay (RIPA) buffer, along with physical disruption such as sonication, or the combination of both, have become the norm for protein extraction from cell membranes, cytoplasm, organelles, and nuclei.

Although RIPA buffer (with 0.1% SDS) or its substitute like NP-40 buffer (without SDS), has been widely used to lyse mammalian cells and tissues, RIPA buffer is not as effective in extracting large proteins as it is in medium and small proteins. To increase the harvest of large proteins, most laboratories combine RIPA buffer with sonication which can physically break down DNA to reduce the viscosity of the lysates. However, sonication has the potential to break down large proteins. Furthermore, to inhibit endogenous enzyme activities, inhibitors need to be added to the RIPA buffer.

IntactProtein™ Cell-Tissue Lysis Kit is formulated to solve these issues. It saves your time by avoiding the extra step of adding protease and phosphatase inhibitors; it can also preserve the structural and signal integrity of the cellular proteins. Further, this product is suitable for extracting all sizes of proteins from both adherent and suspension cells.