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| Reactivity: | Human, Mouse |
| Applications: | WB, IHC, IF/IC, ELISA |
| Host Species: | Rabbit |
| Clonality: | Polyclonal antibody |
| Gene Name: | UbiA prenyltransferase domain containing 1 |
| Gene Symbol: | UBIAD1 |
| Synonyms: | SCCD; TERE1; UBIAD1 |
| Gene ID: | 29914 |
| UniProt ID: | Q9Y5Z9 |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human UBIAD1 (NP_037451.1). |
| Dilution: | WB 1:500-1:2000; IHC 1:100-1:500; IF/IC 1:50-1:200 |
| Purification Method: | Affinity purification |
| Concentration: | 2.18 mg/ml |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
| Documents: | Manual-UBIAD1 antibody |
Background
This gene encodes a protein thought to be involved in cholesterol and phospholipid metabolism. Mutations in this gene are associated with Schnyder crystalline corneal dystrophy.
Images
 | Western blot analysis of lysates from HepG2 cells, using UBIAD1 Rabbit pAb (A14214) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
 | Immunohistochemistry analysis of paraffin-embedded Human colon using UBIAD1 Rabbit pAb (A14214) at dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining. |
 | Immunofluorescence analysis of NIH/3T3 cells using UBIAD1 Rabbit pAb (A14214) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. |
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