LiFluor™ 610 is a bright, far-red rhodamine-based fluorescent dye. LiFluor™ 610 is water-soluable, highly fluorescent and extremely photostable. LiFluor™ 610 can be used as an excellent energy acceptor in fluorescence resonance energy transfer (FRET), or used as a replacement for Alexa Fluor 610.
The NHS ester (or succinimidyl ester) is the most popular amine reactive group for labeling with the primary amines (R-NH2) of proteins, amine-modified oligonucleotides, and other amine-containing molecules. The resulting LiFluor™ conjugate will exhibit brighter fluorescence and greater photostability than the conjugates of other spectrally similar fluorophores.
Key Features
• LiFluor™ fluorescent dye has superior brightness and good photostability.
• The dye is insensitive to pH change and has good water solubility.
• Multiplexing: across spectrum from UV to near-IR.
Specifications
1. Ex/Em: 610/625 nm
2. Extinction coefficient: 100,000
3. Molecular weight: ~1,100
4. Excitation source: 594 nm
Applications
Fluorescent labeling
Component
1. LiFluor™ 610 Fluorescent Dye: 5 mg
Storage
Store at -20°C and protect from light.
Images
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Immunofluorescent analysis of α-tubulin with LiFluor™ 610 conjugated Goat Anti-Mouse IgG (H+L) Antibody. |
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Immunofluorescent analysis of α-tubulin with LiFluor™ 610 conjugated Goat Anti-Rabbit IgG (H+L) Antibody. |
