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ABflo® 488 Rabbit anti-Human CD300c mAb (100 T)
| Reactivity: | Human |
| Applications: | IF/ICC, FC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal Antibody |
| Conjugation: | ABflo® 488. Ex:491nm. Em:516nm. |
| Gene Name: | CD300c molecule |
| Gene Symbol: | CD300c |
| Synonyms: | LIR; CLM-6; CMRF35; IGSF16; CMRF-35; CMRF35A; CMRF-35A; CMRF35A1; CMRF35-A1 |
| Gene ID: | 10871 |
| UniProt ID: | Q08708 |
| Clone ID: | 2J8X4 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 21-183 of human CD300C (NP_006669.1). |
| Dilution: | IF/ICC,1:50 - 1:200|FC,5 μl per 10^6 cells in 100 μl volume |
| Purification Method: | Affinity purification |
| Concentration: | 0.04 mg/mL |
| Buffer: | PBS with 0.03% proclin300,0.2% BSA, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-CD300c Antibody |
Background
The CMRF35 antigen, which was identified by reactivity with a monoclonal antibody, is present on monocytes, neutrophils, and some T and B lymphocytes (Jackson et al., 1992 [PubMed 1349532]).
Images
 | Confocal imaging of 293T cells transfected with CD300c using ABflo® 488 Rabbit anti-Human CD300c mAb (A24097, dilution 1:200). DAPI was used for nuclear staining (Blue). Objective: 100x. |
 | Flow cytometry: 1×10^6 293F cells (negative control,left) and 293F (Transfection,right) cells were surface-stained with ABflo® 488 Rabbit anti-Human CD300c mAb (A24097,5 μl/Test,orange line) or ABflo® 488 Rabbit IgG isotype control (A22069,5 μl/Test,blue line). Non-fluorescently stained cells were used as blank control (red line). |
 | Flow cytometry: 1×10^6 293F (Transfection) cells were surface-stained with ABflo® 488 Rabbit IgG isotype control (A22069,5 μl/Test,left) or ABflo® 488 Rabbit anti-Human CD300c mAb (A24097,5 μl/Test,right). |
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