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| Reactivity: | Human |
| Applications: | FC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal Antibody |
| Conjugation: | ABflo® 488. Ex:491nm. Em:516nm. |
| Gene Name: | CD58 molecule |
| Gene Symbol: | CD58 |
| Synonyms: | ag3; LFA3; LFA-3 |
| Gene ID: | 965 |
| UniProt ID: | P19256 |
| Clone ID: | 7C1C7 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 29-215 of human CD58 (NP_001770.1). |
| Dilution: | FC,5 μl per 10^6 cells in 100 μl volume |
| Purification Method: | Affinity purification |
| Concentration: | 0.02 mg/mL |
| Buffer: | PBS with 0.03% proclin300,0.2% BSA, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-CD58 Antibody |
Background
This gene encodes a member of the immunoglobulin superfamily. The encoded protein is a ligand of the T lymphocyte CD2 protein, and functions in adhesion and activation of T lymphocytes. The protein is localized to the plasma membrane. Alternatively spliced transcript variants have been described.
Images
![]() | Flow cytometry:1×10^6 A204 cells (Low Expression,left) and MCF7 cells (right) were surface-stained with ABflo® 488 Rabbit anti-Human CD58 mAb(A22512,5 μl/Test,orange line) or ABflo® 488 Rabbit IgG isotype control (A22069,5 μl/Test,blue line). Non-fluorescently stained cells were used as blank control (red line). |
![]() | Flow cytometry:1×10^6 MCF7 cells were surface-stained with ABflo® 488 Rabbit IgG isotype control (A22069,5 μl/Test,left) or ABflo® 488 Rabbit anti-Human CD58 mAb(A22512,5 μl/Test,right). |
![]() | Flow cytometry:1×10^6 A204 cells(Low Expression) were intracellularly-stained with ABflo® 488 Rabbit IgG isotype control (A22069,5 μl/Test,left) or ABflo® 488 Rabbit anti-Human CD58 mAb(A22512,5 μl/Test,right). |
![]() | Flow cytometry:1×10^6 Human PBMC were surface-stained with ABflo® 488 Rabbit anti-Human CD58 mAb(A22512,5 μl/Test,orange line) or ABflo® 488 Rabbit IgG isotype control (A22069,5 μl/Test,blue line).Non-fluorescently stained Human PBMC was used as blank control (red line). |
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