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ABflo® 488 Rabbit anti-Human CD79b/Igβ mAb (100 T)
| Reactivity: | Human |
| Applications: | IF/ICC, FC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal Antibody |
| Conjugation: | ABflo® 488. Ex:491nm. Em:516nm. |
| Gene Name: | CD79b molecule |
| Gene Symbol: | CD79b |
| Synonyms: | B29; IGB; AGM6; Igbeta |
| Gene ID: | 974 |
| UniProt ID: | P40259 |
| Clone ID: | 10L9A4 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 29-159 of human CD79b/Igβ(NP_000617.1). |
| Dilution: | IF/ICC,1:50 - 1:200|FC,5 μl per 10^6 cells in 100 μl volume |
| Purification Method: | Affinity purification |
| Concentration: | 0.04 mg/mL |
| Buffer: | PBS with 0.03% proclin300,0.2% BSA, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-CD79b Antibody |
Background
The B lymphocyte antigen receptor is a multimeric complex that includes the antigen-specific component, surface immunoglobulin (Ig). Surface Ig non-covalently associates with two other proteins, Ig-alpha and Ig-beta, which are necessary for expression and function of the B-cell antigen receptor. This gene encodes the Ig-beta protein of the B-cell antigen component. Alternatively spliced transcript variants encoding different isoforms have been described.
Images
 | Confocal imaging of Daudi cells using ABflo® 488 Rabbit anti-Human CD79b/Igβ mAb (A23801, dilution 1:200). DAPI was used for nuclear staining (Blue). Objective: 100x. |
 | Flow cytometry: 1×10^6 HAP1 cells(negative controll,left) and Daudi cells (right) were surface-stained with ABflo® 488 Rabbit anti-Human CD79b/Igβ mAb(A23801,5 μl/Test,orange line) or ABflo® 488 Rabbit IgG isotype control (A22069,5 μl/Test,blue line).Non-fluorescently stained cells were used as blank control (red line). |
 | Flow cytometry:1×10^6 Daudi cells were surface-stained with ABflo® 488 Rabbit IgG isotype control (A22069,5 μl/Test,left) or ABflo® 488 Rabbit anti-Human CD79b/Igβ mAb(A23801,5 μl/Test,right). |
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