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ABflo® 488 Rabbit anti-Mouse CD24 mAb (100 T)
| Reactivity: | Mouse |
| Applications: | IF/ICC, FC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal Antibody |
| Conjugation: | ABflo® 488. Ex:491nm. Em:516nm. |
| Gene Name: | CD24a antigen |
| Gene Symbol: | Cd24a |
| Synonyms: | HSA; Cd24; Ly-52; nectadrin |
| Gene ID: | 12484 |
| UniProt ID: | P24807 |
| Clone ID: | 6V10P3 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 27-53 of mouse CD24 (NP_033976.1). |
| Dilution: | IF/ICC,1:50 - 1:200|FC,5 μl per 10^6 cells in 100 μl volume |
| Purification Method: | Affinity purification |
| Concentration: | 0.01 mg/mL |
| Buffer: | PBS with 0.03% proclin300,0.2% BSA, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-Cd24a Antibody |
Background
CD24, also know as heat stable antigen HSA, is a P-selectin ligand involved in adhesion. It is a GPI-anchored glycoprotein expressed on many types of cells, including hematopoietic cells, neural cells, and epithelial cells. CD24 is widely used to delineate stages of lymphocyte development . It also binds to Siglec-10 in humans or Siglec-G in mice . CD24 is frequently used as a marker to identify and isolate cancer stem cells in various cancer types .
Images
 | Confocal imaging of Neuro-2a cells using ABflo® 488 Rabbit anti-Mouse CD24 mAb (A25470, dilution 1:50). DAPI was used for nuclear staining (Blue). Objective: 100x. |
 | Confocal imaging of paraffin-embedded Mouse spleen tissue using ABflo® 488 Rabbit anti-Mouse CD24 mAb (A25470, dilution 1:50). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x. |
 | Flow cytometry: 1×10^6 RAW 264.7 cells (Low Expression,left) and C57BL/6 mouse Splenocytes (right) were surface-stained with ABflo® 488 Rabbit anti-Mouse CD24 mAb (A25470,5 μl/Test,orange line) or ABflo® 488 Rabbit IgG isotype control (A22069,5 μl/Test,blue line). Non-fluorescently stained cells were used as blank control (red line). |
 | Flow cytometry: 1×10^6 C57BL/6 mouse Splenocytes were surface-stained with ABflo® 488 Rabbit IgG isotype control (A22069,5 μl/Test,left) or ABflo® 488 Rabbit anti-Mouse CD24 mAb (A25470,5 μl/Test,right). |
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