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ABflo® 488 Rabbit anti-Mouse CD28 mAb (100 T)
| Reactivity: | Mouse |
| Applications: | FC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal Antibody |
| Conjugation: | ABflo® 488. Ex:491nm. Em:516nm. |
| Gene Name: | CD28 antigen |
| Gene Symbol: | Cd28 |
| Synonyms: | N/A |
| Gene ID: | 12487 |
| UniProt ID: | P31041 |
| Clone ID: | 7R0V2 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 20-149 of mouse CD28 (NP_031668.3). |
| Dilution: | FC,5 μl per 10^6 cells in 100 μl volume |
| Purification Method: | Affinity purification |
| Concentration: | 0.04 mg/mL |
| Buffer: | PBS with 0.03% proclin300,0.2% BSA, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-Cd28 Antibody |
Background
Enables protein kinase binding activity. Acts upstream of or within several processes, including positive regulation of immune response; positive regulation of intracellular signal transduction; and regulation of lymphocyte activation. Located in external side of plasma membrane and immunological synapse. Human ortholog(s) of this gene implicated in multiple sclerosis and type 1 diabetes mellitus. Orthologous to human CD28 (CD28 molecule).
Images
 | Flow cytometry: 1×10^6 NIH/3T3 cells (negative control,left) and C57BL/6 mouse Splenocytes (right) were surface-stained with ABflo® 488 Rabbit anti-Mouse CD28 mAb (A25056,5 μl/Test,orange line) or ABflo® 488 Rabbit IgG isotype control (A22069,5 μl/Test,blue line). Non-fluorescently stained cells were used as blank control (red line). |
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