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ABflo® 488 Rabbit anti-Mouse CD47 mAb (100 T)
| Reactivity: | Mouse |
| Applications: | FC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal Antibody |
| Conjugation: | ABflo® 488. Ex:491nm. Em:516nm. |
| Gene Name: | CD47 antigen (Rh-related antigen, integrin-associated signal transducer) |
| Gene Symbol: | Cd47 |
| Synonyms: | IAP; Itgp; 9130415E20Rik; B430305P08Rik |
| Gene ID: | 16423 |
| UniProt ID: | Q61735 |
| Clone ID: | 10S9F6 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 19-135 of mouse CD47 (NP_034711.1). |
| Dilution: | FC,5 μl per 10^6 cells in 100 μl volume |
| Purification Method: | Affinity purification |
| Concentration: | 0.01 mg/mL |
| Buffer: | PBS with 0.03% proclin300,0.2% BSA, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-Cd47 antibody |
Background
Predicted to enable protein binding activity involved in heterotypic cell-cell adhesion and thrombospondin receptor activity. Involved in regulation of nitric oxide biosynthetic process. Acts upstream of or within several processes, including monocyte aggregation; opsonization; and positive regulation of phagocytosis. Located in extracellular exosome. Is integral component of plasma membrane. Is expressed in several structures, including alimentary system; central nervous system; genitourinary system; hemolymphoid system gland; and liver and biliary system. Orthologous to human CD47 (CD47 molecule).
Images
 | Flow cytometry: 1×10^6 C57 BL/6 mouse Splenocytes were surface-stained with ABflo® 488 Rabbit anti-Mouse CD47 mAb (A24377,5 μl/Test,orange line) or ABflo® 488 Rabbit IgG isotype control (A22069,5 μl/Test,blue line). Non-fluorescently stained C57 BL/6 mouse Splenocytes were used as blank control (red line). |
 | Flow cytometry: 1×10^6 Hep G2 cells (negative control) were surface-stained with ABflo® 488 Rabbit anti-Mouse CD47 mAb (A24377,5 μl/Test,orange line) or ABflo® 488 Rabbit IgG isotype control (A22069,5 μl/Test,blue line). Non-fluorescently stained Hep G2 cells were used as blank control (red line). |
 | Flow cytometry: 1×10^6 C57 BL/6 mouse Splenocytes were surface-stained with ABflo® 488 Rabbit IgG isotype control (A22069,5 μl/Test,left) or ABflo® 488 Rabbit anti-Mouse CD47 mAb (A24377,5 μl/Test,right). |
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