ABflo® 594 Rabbit anti-Human Phospho-PI3KP85α-Y467+P85β-Y464+P55γ-Y199 mAb (100 T)

ABflo® 594 Rabbit anti-Human Phospho-PI3KP85α-Y467+P85β-Y464+P55γ-Y199 mAb (100 T)

Brand: Abclonal
Cat. #: AP1489
Availability: In Stock
$478.00
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Reactivity: Human    
Applications: FC (intra)    
Host Species: Rabbit    
Isotype: IgG    
Clonality: Monoclonal Antibody    
Conjugation: ABflo® 594. Ex:588nm. Em:604nm.
Gene Name: phosphoinositide-3-kinase regulatory subunit 1
Gene Symbol: PIK3R1
Synonyms: p85; AGM7; GRB1; IMD36; p85alpha; p85-ALPHA
Gene ID: 5295/5296/8503
UniProt ID: P27986/O00459/Q92569
Clone ID: 1H3P7
Immunogen: A synthetic phosphorylated peptide around Y467 of human PI3KP85α (NP_852664.1).
Dilution: FC (intra),5 μl per 10^6 cells in 100 μl volume
Purification Method: Affinity purification
Concentration: 0.04 mg/mL
Buffer: PBS with 0.03% proclin300,0.2% BSA, pH7.3.
Storage: Store at -20°C. Avoid freeze / thaw cycles.
Documents: Manual-PIK3R1 antibody




Background

Phosphatidylinositol 3-kinase phosphorylates the inositol ring of phosphatidylinositol at the 3-prime position. The enzyme comprises a 110 kD catalytic subunit and a regulatory subunit of either 85, 55, or 50 kD. This gene encodes the 85 kD regulatory subunit. Phosphatidylinositol 3-kinase plays an important role in the metabolic actions of insulin, and a mutation in this gene has been associated with insulin resistance. Alternative splicing of this gene results in four transcript variants encoding different isoforms. [provided by RefSeq, Jun 2011]




Images

PIK3R1 antibodyFlow cytometry: 1×10^6 293T cells (negative control,left) and 293T (Transfection,right) cells were intracellularly-stained with ABflo® 594 Rabbit anti-Human Phospho-PI3KP85α-Y467+P85β-Y464+P55γ-Y199 mAb (AP1489,5 μl/Test,orange line) or ABflo® 594 Rabbit IgG isotype control (A23821,5 μl/Test,blue line). Non-fluorescently stained cells were used as blank control (red line).
Flow cytometry: 1×10^6 293T (Transfection) cells were intracellularly-stained with ABflo® 594 Rabbit IgG isotype control (A23821,5 μl/Test,left) or ABflo® 594 Rabbit anti-Human Phospho-PI3KP85α-Y467+P85β-Y464+P55γ-Y199 mAb (AP1489,5 μl/Test,right).



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