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ABflo® 594 Rabbit anti-Mouse CD326/EpCAM mAb (100 T)
| Reactivity: | Mouse |
| Applications: | IF/ICC,FC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal Antibody |
| Conjugation: | ABflo® 594. Ex:588nm. Em:604nm. |
| Gene Name: | epithelial cell adhesion molecule |
| Gene Symbol: | Epcam |
| Synonyms: | EGP; Ly74; gp40; CD326; EGP-2; TROP1; Egp314; Ep-CAM; EpCAM1; Tacsd1; GA733-2; Tacstd1 |
| Gene ID: | 17075 |
| UniProt ID: | Q99JW5 |
| Clone ID: | 1C1Y6 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 24-266 of mouse CD326/EpCAM (NP_032558.2). |
| Dilution: | IF/ICC,1:50 - 1:200|FC,5 μl per 10^6 cells in 100 μl volume |
| Purification Method: | Affinity purification |
| Concentration: | 0.01 mg/mL |
| Buffer: | PBS with 0.03% proclin300,0.2% BSA, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-Epcam antibody |
Background
Predicted to enable cadherin binding activity involved in cell-cell adhesion. Acts upstream of or within ureteric bud development. Located in cell surface. Is expressed in several structures, including alimentary system; central nervous system; genitourinary system; respiratory system; and sensory organ. Used to study congenital diarrhea 5 with tufting enteropathy. Human ortholog(s) of this gene implicated in congenital diarrhea 5 with tufting enteropathy and hereditary nonpolyposis colorectal cancer type 8. Orthologous to human EPCAM (epithelial cell adhesion molecule).
Images
 | Confocal imaging of paraffin-embedded Mouse colon tissue using ABflo® 594 Rabbit anti-Mouse CD326/EpCAM mAb (A25347, dilution 1:100). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x. |
 | Confocal imaging of paraffin-embedded Mouse small intestine tissue using ABflo® 594 Rabbit anti-Mouse CD326/EpCAM mAb (A25347, dilution 1:100). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x. |
 | Confocal imaging of paraffin-embedded Rat small intestine tissue using ABflo® 594 Rabbit anti-Mouse CD326/EpCAM mAb (A25347, dilution 1:100). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x. |
 | Flow cytometry: 1×10^6 293T cells (negative control,left) and 293T (Transfection,right) cells were surface-stained with ABflo® 594 Rabbit anti-Mouse CD326/EpCAM mAb (A25347,5 μl/Test,orange line) or ABflo® 594 Rabbit IgG isotype control (A23821,5 μl/Test,blue line). Non-fluorescently stained cells were used as blank control (red line). |
 | Flow cytometry: 1×10^6 293T (Transfection) cells were surface-stained with ABflo® 594 Rabbit IgG isotype control (A23821,5 μl/Test,left) or ABflo® 594 Rabbit anti-Mouse CD326/EpCAM mAb (A25347,5 μl/Test,right). |
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