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ABflo® 647 Rabbit anti-4-Hydroxynonenal mAb (100 T)
| Applications: | FC (intra) |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal Antibody |
| Conjugation: | ABflo® 647. Ex:648nm. Em:664nm. |
| Gene Symbol: | 4-HNE |
| Synonyms: | 4-HNE |
| Immunogen: | Chemical compounds corresponding to 4-Hydroxynonenal. |
| Dilution: | FC (intra),5 μl per 10^6 cells in 100 μl volume |
| Purification Method: | Affinity purification |
| Concentration: | 0.04 mg/ml |
| Buffer: | PBS with 0.09% Sodium azide,0.2% BSA, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-4-HNE antibody |
Background
4-hydroxy-2-nonenal (4-hydroxynonenal, 4-HNE) is a highly reactive aldehyde generated by the exposure of polyunsaturated fatty acids to peroxides and reactive oxygen species (ROS). It non-enzymatically forms stable protein adducts with histidine, lysine, and cysteine side chains that have been used as biomarkers for oxidative damage in cells. Conditions where 4-HNE immunoreactivity has been observed include include inflammation, neurodegenerative diseases, and ischemic damage to the heart and brain.
Images
 | Flow cytometry: 1×10^6 HeLa cells (negative control,left) and HeLa cells (treated with 4-Hydroxynonenal,right) were intracellularly-stained with ABflo® 647 Rabbit anti-4-Hydroxynonenal mAb (A26081,5 μl/Test,orange line) or ABflo® 647 Rabbit IgG isotype control (A22070,5 μl/Test,blue line). Non-fluorescently stained cells were used as blank control (red line). |
 | Flow cytometry: 1×10^6 HeLa cells (treated with 4-Hydroxynonenal) were intracellularly-stained with ABflo® 647 Rabbit IgG isotype control (A22070,5 μl/Test,left) or ABflo® 647 Rabbit anti-4-Hydroxynonenal mAb (A26081,5 μl/Test,right). |
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