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ABflo® 647 Rabbit anti-Human BTN3A1/2/3 mAb (100 T)
| Reactivity: | Human |
| Applications: | FC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal Antibody |
| Conjugation: | ABflo® 647. Ex:648nm. Em:664nm. |
| Gene Name: | butyrophilin subfamily 3 member A3 |
| Gene Symbol: | BTN3A3 |
| Synonyms: | BTF3; BTN3.3 |
| Gene ID: | 11119/11118/10384 |
| UniProt ID: | O00481/P78410/O00478 |
| Clone ID: | 4E6B0 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 470-630 of human BTN3A1(NP_008979.3). |
| Dilution: | FC,5 μl per 10^6 cells in 100 μl volume |
| Purification Method: | Affinity purification |
| Concentration: | 0.01 mg/mL |
| Buffer: | PBS with 0.03% proclin300,0.2% BSA, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-BTN3A3 antibody |
Background
The butyrophilin (BTN) genes are a group of major histocompatibility complex (MHC)-associated genes that encode type I membrane proteins with 2 extracellular immunoglobulin (Ig) domains and an intracellular B30.2 (PRYSPRY) domain. Three subfamilies of human BTN genes are located in the MHC class I region: the single-copy BTN1A1 gene (MIM 601610) and the BTN2 (e.g., BTN2A1; MIM 613590) and BTN3 (e.g., BNT3A1) genes, which have undergone tandem duplication, resulting in 3 copies of each (summary by Smith et al., 2010 [PubMed 20208008]).
Images
 | Flow cytometry: 1×10^6 MCF7 cells (Low Expression,left) and U266 cells (right) were surface-stained with ABflo® 647 Rabbit anti-Human BTN3A1/2/3 mAb (A24822,5 μl/Test,orange line) or ABflo® 647 Rabbit IgG isotype control (A22070,5 μl/Test,blue line). Non-fluorescently stained cells were used as blank control (red line). |
 | Flow cytometry: 1×10^6 U266 cells were surface-stained with ABflo® 647 Rabbit IgG isotype control (A22070,5 μl/Test,left) or ABflo® 647 Rabbit anti-Human BTN3A1/2/3 mAb (A24822,5 μl/Test,right). |
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