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ABflo® 647 Rabbit anti-Human CD34 mAb (100 T)
| Reactivity: | Human |
| Applications: | IF/ICC, FC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal Antibody |
| Conjugation: | ABflo® 647. Ex:648nm. Em:664nm. |
| Gene Name: | CD34 molecule |
| Gene Symbol: | CD34 |
| Synonyms: | Hematopoietic progenitor cell antigen CD34; CD34 |
| Gene ID: | 947 |
| UniProt ID: | P28906 |
| Clone ID: | 0F4S1 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 170-290 of human CD34 (NP_001020280.1). |
| Dilution: | IF/ICC,1:50 - 1:200|FC,5 μl per 10^6 cells in 100 μl volume |
| Purification Method: | Affinity purification |
| Concentration: | 0.04 mg/mL |
| Buffer: | PBS with 0.03% proclin300,0.2% BSA, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-CD34 Antibody |
Background
The protein encoded by this gene may play a role in the attachment of stem cells to the bone marrow extracellular matrix or to stromal cells. This single-pass membrane protein is highly glycosylated and phosphorylated by protein kinase C. Two transcript variants encoding different isoforms have been found for this gene.
Images
 | Confocal imaging of TF-1 cells using ABflo® 647 Rabbit anti-Human CD34 mAb (A25894, dilution 1:100) (Red). DAPI was used for nuclear staining (Blue). Objective: 100x. |
 | Flow cytometry: 1×10^6 HeLa cells (negative control,left) and MOLT-4 cells (right) were surface-stained with ABflo® 647 Rabbit anti-Human CD34 mAb (A25894,5 μl/Test,orange line) or ABflo® 647 Rabbit IgG isotype control (A22070,5 μl/Test,blue line). Non-fluorescently stained cells were used as blank control (red line). |
 | Flow cytometry: 1×10^6 MOLT-4 cells were surface-stained with ABflo® 647 Rabbit IgG isotype control (A22070,5 μl/Test,left) or ABflo® 647 Rabbit anti-Human CD34 mAb (A25894,5 μl/Test,right). |
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