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ABflo® 647 Rabbit anti-Human CD47 mAb (100 T)
| Reactivity: | Human |
| Applications: | FC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal Antibody |
| Conjugation: | ABflo® 647. Ex:648nm. Em:664nm. |
| Gene Name: | CD47 molecule |
| Gene Symbol: | CD47 |
| Synonyms: | IAP; OA3; MER6 |
| Gene ID: | 961 |
| UniProt ID: | Q08722 |
| Clone ID: | 1Q0R4 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 19-139 of human CD47 (NP_001768.1). |
| Dilution: | FC,5 μl per 10^6 cells in 100 μl volume |
| Purification Method: | Affinity purification |
| Concentration: | 0.04 mg/mL |
| Buffer: | PBS with 0.03% proclin300,0.2% BSA, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-CD47 Antibody |
Background
This gene encodes a membrane protein, which is involved in the increase in intracellular calcium concentration that occurs upon cell adhesion to extracellular matrix. The encoded protein is also a receptor for the C-terminal cell binding domain of thrombospondin, and it may play a role in membrane transport and signal transduction. This gene has broad tissue distribution, and is reduced in expression on Rh erythrocytes. Alternatively spliced transcript variants have been found for this gene.
Images
 | Flow cytometry:1×10^6 HepG2 cells (Low Expression, Left) and MOLT-4 cells(Right) were surface-stained with ABflo® 647 Rabbit anti-Human CD47 mAb(A24301,5 μl/Test,orange line) or ABflo® 647 Rabbit IgG isotype control (A22070,5 μl/Test,blue line). Non-fluorescently stained cells were used as blank control (red line). |
 | Flow cytometry:1×10^6 MOLT-4 cells were surface-stained with ABflo® 647 Rabbit IgG isotype control (A22070,5 μl/Test,left) or ABflo® 647 Rabbit anti-Human CD47 mAb(A24301,5 μl/Test,right). |
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