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ABflo® 647 Rabbit anti-Human EGFR mAb (100 T)
| Reactivity: | Human |
| Applications: | IF/ICC, FC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal Antibody |
| Conjugation: | ABflo® 647. Ex:648nm. Em:664nm. |
| Gene Name: | Epidermal growth factor receptor |
| Gene Symbol: | EGFR |
| Synonyms: | ERBB; ERRP; HER1; mENA; ERBB1; NNCIS; PIG61; NISBD2 |
| Gene ID: | 1956 |
| UniProt ID: | P00533 |
| Clone ID: | 10W6W10 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 335-525 of human EGFR(NP_005219.2). |
| Dilution: | IF/ICC,1:50 - 1:200|FC,5 μl per 10^6 cells in 100 μl volume |
| Purification Method: | Affinity purification |
| Concentration: | 0.04 mg/mL |
| Buffer: | PBS with 0.03% proclin300,0.2% BSA, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-EGFR Antibody |
Background
The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer.
Images
 | Confocal imaging of A-431 cells using ABflo® 647 Rabbit anti-Human EGFR mAb (A24266, dilution 1:25). DAPI was used for nuclear staining (Blue). Objective: 100x. |
 | Flow cytometry: 1×10^6 Jurkat cells (negative control,left) and A-431 cells (right) were surface-stained with ABflo® 647 Rabbit anti-Human EGFR mAb(A24266,5 μl/Test,orange line) or ABflo® 647 Rabbit IgG isotype control (A22070,5 μl/Test,blue line).Non-fluorescently stained cells were used as blank control (red line). |
 | Flow cytometry:1×10^6 A-431 cells were surface-stained with ABflo® 647 Rabbit IgG isotype control (A22070,5 μl/Test,left) or ABflo® 647 Rabbit anti-Human EGFR mAb(A24266,5 μl/Test,right). |
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