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ABflo® 647 Rabbit anti-Human/Monkey CD16 mAb (100 T)
| Reactivity: | Human, Monkey |
| Applications: | FC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal Antibody |
| Conjugation: | ABflo® 647. Ex:648nm. Em:664nm. |
| Gene Name: | Fc gamma receptor IIIa |
| Gene Symbol: | FCGR3A |
| Synonyms: | CD16; FCG3; CD16A; FCGR3; IGFR3; IMD20; FCR-10; FCRIII; CD16-II; FCGRIII; FCRIIIA; FcGRIIIA |
| Gene ID: | 2214/2215 |
| UniProt ID: | P08637/O75015 |
| Clone ID: | 1E8H4 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 20-208 of human FCGR3A/FCGR3B (P08637/O75015). |
| Dilution: | FC,5 μl per 10^6 cells in 100 μl volume |
| Purification Method: | Affinity purification |
| Concentration: | 0.04 mg/mL |
| Buffer: | PBS with 0.03% proclin300,0.2% BSA, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-FCGR3A antibody |
Background
This gene encodes a receptor for the Fc portion of immunoglobulin G, and it is involved in the removal of antigen-antibody complexes from the circulation, as well as other responses, including antibody dependent cellular mediated cytotoxicity and antibody dependent enhancement of virus infections. This gene (FCGR3A) is highly similar to another nearby gene (FCGR3B) located on chromosome 1. The receptor encoded by this gene is expressed on natural killer (NK) cells as an integral membrane glycoprotein anchored through a transmembrane peptide, whereas FCGR3B is expressed on polymorphonuclear neutrophils (PMN) where the receptor is anchored through a phosphatidylinositol (PI) linkage. Mutations in this gene are associated with immunodeficiency 20, and have been linked to susceptibility to recurrent viral infections, susceptibility to systemic lupus erythematosus, and alloimmune neonatal neutropenia. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Images
 | Flow cytometry:1×10^6 293F cells (negative control,left) and 293F-CD16b (Transfection,right) cells were surface-stained with ABflo® 647 Rabbit anti-Human/Monkey CD16 mAb (A23400,5 μl/Test,orange line) or ABflo® 647 Rabbit IgG isotype control (A22070,5 μl/Test,blue line). Non-fluorescently stained cells was used as blank control (red line). |
 | Flow cytometry:1×10^6 293F-CD16a (Transfection) cells were surface-stained with ABflo® 647 Rabbit IgG isotype control (A22070,5 μl/Test,left) or ABflo® 647 Rabbit anti-Human/Monkey CD16 mAb (A23400,5 μl/Test,right). |
 | Flow cytometry:1×10^6 293F-CD16b (Transfection) cells were surface-stained with ABflo® 647 Rabbit IgG isotype control (A22070,5 μl/Test,left) or ABflo® 647 Rabbit anti-Human/Monkey CD16 mAb (A23400,5 μl/Test,right). |
 | Flow cytometry:1×10^6 Human PBMC were surface-stained with ABflo® 647 Rabbit anti-Human/Monkey CD16 mAb (A23400,5 μl/Test,orange line) or ABflo® 647 Rabbit IgG isotype control (A22070,5 μl/Test,blue line). Non-fluorescently stained cells were used as blank control (red line). Cells in the lymphocyte gate were used for analysis. |
 | Flow cytometry:1×10^6 Human PBMC were surface-stained with ABflo® 647 Rabbit IgG isotype control (A22070,5 μl/Test,left) or ABflo® 647 Rabbit anti-Human/Monkey CD16 mAb (A23400,5 μl/Test,right). Cells in the lymphocyte gate were used for analysis. |
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