Reactivity: | Human |
Applications: | FC (intra) |
Host Species: | Rabbit |
Isotype: | IgG |
Clonality: | Monoclonal Antibody |
Conjugation: | ABflo® 647. Ex:648nm. Em:664nm. |
Gene Name: | phosphoinositide-3-kinase regulatory subunit 1 |
Gene Symbol: | PIK3R1 |
Synonyms: | p85; AGM7; GRB1; IMD36; p85alpha; p85-ALPHA |
Gene ID: | 5295/5296/8503 |
UniProt ID: | P27986/O00459/Q92569 |
Clone ID: | 5N6V3 |
Immunogen: | A synthetic phosphorylated peptide around Y467 of human PI3KP85α (NP_852664.1). |
Dilution: | FC (intra),5 μl per 10^6 cells in 100 μl volume |
Purification Method: | Affinity purification |
Concentration: | 0.04 mg/mL |
Buffer: | PBS with 0.03% proclin300,0.2% BSA, pH7.3. |
Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
Documents: | Manual-PIK3R1 antibody |
Background
Phosphatidylinositol 3-kinase phosphorylates the inositol ring of phosphatidylinositol at the 3-prime position. The enzyme comprises a 110 kD catalytic subunit and a regulatory subunit of either 85, 55, or 50 kD. This gene encodes the 85 kD regulatory subunit. Phosphatidylinositol 3-kinase plays an important role in the metabolic actions of insulin, and a mutation in this gene has been associated with insulin resistance. Alternative splicing of this gene results in four transcript variants encoding different isoforms. [provided by RefSeq, Jun 2011]
Images
![]() | Flow cytometry: 1×10^6 293T cells (negative control,left) and 293T (Transfection,right) cells were intracellularly-stained with ABflo® 647 Rabbit anti-Human Phospho-PI3KP85α-Y467+P85β-Y464+P55γ-Y199 mAb (AP1488,5 μl/Test,orange line) or ABflo® 647 Rabbit IgG isotype control (A22070,5 μl/Test,blue line). Non-fluorescently stained cells were used as blank control (red line). |
![]() | Flow cytometry: 1×10^6 293T (Transfection) cells were intracellularly-stained with ABflo® 647 Rabbit IgG isotype control (A22070,5 μl/Test,left) or ABflo® 647 Rabbit anti-Human Phospho-PI3KP85α-Y467+P85β-Y464+P55γ-Y199 mAb (AP1488,5 μl/Test,right). |
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