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ABflo® 647 Rabbit anti-Human TNF-α mAb (100 T)
| Reactivity: | Human |
| Applications: | FC (intra) |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal Antibody |
| Conjugation: | ABflo® 647. Ex:648nm. Em:664nm. |
| Gene Name: | tumor necrosis factor |
| Gene Symbol: | TNF |
| Synonyms: | DIF; TNFA; IMD127; TNFSF2; TNLG1F; TNF-alpha |
| Gene ID: | 7124 |
| UniProt ID: | P01375 |
| Clone ID: | 3I7I6 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 77-233 of human TNF-α (NP_002344.2). |
| Dilution: | FC (intra),5 μl per 10^6 cells in 100 μl volume |
| Purification Method: | Affinity purification |
| Concentration: | 0.04 mg/mL |
| Buffer: | PBS with 0.03% proclin300,0.2% BSA, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-TNF Antibody |
Background
This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, psoriasis, rheumatoid arthritis ankylosing spondylitis, tuberculosis, autosomal dominant polycystic kidney disease, and cancer. Mutations in this gene affect susceptibility to cerebral malaria, septic shock, and Alzheimer disease. Knockout studies in mice also suggested the neuroprotective function of this cytokine.
Images
 | Flow cytometry:1×10^6 293F cells (negative control,Left) and 293F(Transfection,right) cells were intracellularly-stained with ABflo® 647 Rabbit anti-Human TNF-α mAb(A22782,5 μl/Test,orange line) or ABflo® 647 Rabbit IgG isotype control (A22070,5 μl/Test,blue line). Non-fluorescently stained cells were used as blank control (red line). |
 | Flow cytometry:1×10^6 293F(Transfection) cells were intracellularly-stained with ABflo® 647 Rabbit IgG isotype control (A22070,5 μl/Test,left) or ABflo® 647 Rabbit anti-Human TNF-α mAb(A22782,5 μl/Test,right). |
 | Flow cytometry:1×10^6 Human PBMC were intracellularly-stained with ABflo® 647 Rabbit anti-Human TNF-α mAb(A22782,5 μl/Test,orange line) or ABflo® 647 Rabbit IgG isotype control (A22070,5 μl/Test,blue line). Non-fluorescently stained cells were used as blank control (red line). |
 | Flow cytometry:1×10^6 Human PBMC were intracellularly-stained with ABflo® 647 Rabbit IgG isotype control (A22070,5 μl/Test,left) or ABflo® 647 Rabbit anti-Human TNF-α mAb(A22782,5 μl/Test,right). |
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