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ABflo® 647 Rabbit anti-Mouse/Human CD55 mAb (100 T)
| Reactivity: | Human, Mouse |
| Applications: | FC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal Antibody |
| Conjugation: | ABflo® 647. Ex:648nm. Em:664nm. |
| Gene Name: | CD55 molecule, decay accelerating factor for complement |
| Gene Symbol: | Cd55 |
| Synonyms: | Daf; Daf1; Daf-GPI; GPI-DAF |
| Gene ID: | 13136 |
| UniProt ID: | Q61475 |
| Clone ID: | 7K6U8 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 35-361 of mouse CD55/DAF（NP_034146.2）. |
| Dilution: | FC,5 μl per 10^6 cells in 100 μl volume |
| Purification Method: | Affinity purification |
| Concentration: | 0.04 mg/mL |
| Buffer: | PBS with 0.03% proclin300,0.2% BSA, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-Cd55 antibody |
Background
This gene encodes an inhibitor of both the classical and the alternative pathways of complement activation. The encoded preproprotein undergoes post-translational processing to generate a mature polypeptide anchored to the plasma membrane via a glycosylphosphatidylinositol moiety. Erythrocytes from mice deficient in the encoded protein exhibit impaired regulation of complement activation resulting in enhanced complement deposition. Mice lacking the encoded protein exhibit enhanced susceptibility to experimentally induced myasthenia gravis. This gene is located adjacent to a closely related gene on chromosome 1.
Images
 | Flow cytometry: 1×10^6 C57BL/6 mouse Splenocytes were surface-stained with ABflo® 647 Rabbit anti-Human/Mouse CD55/DAF mAb(A23898,5 μl/Test,orange line) or ABflo® 647 Rabbit IgG isotype control (A22070,5 μl/Test,blue line).Non-fluorescently stained C57BL/6 mouse Splenocytes were used as blank control (red line). |
 | Flow cytometry: 1×10^6 SH-SY5Y cells(negative control,left) and Hela cells(right) were surface-stained with ABflo® 647 Rabbit anti-Human/Mouse CD55/DAF mAb(A23898,5 μl/Test,orange line) or ABflo® 647 Rabbit IgG isotype control (A22070,5 μl/Test,blue line).Non-fluorescently stained cells were used as blank control (red line). ; Flow cytometry: 1×10^6 C57BL/6 mouse Splenocytes were surface-stained with ABflo® 647 Rabbit anti-Human/Mouse CD55/DAF mAb(A23898,5 μl/Test,orange line) or ABflo® 647 Rabbit IgG isotype control (A22070,5 μl/Test,blue line).Non-fluorescently stained C57BL/6 mouse Splenocytes were used as blank control (red line). |
 | Flow cytometry:1×10^6 C57BL/6 mouse Splenocytes were surface-stained with ABflo® 647 Rabbit IgG isotype control (A22070,5 μl/Test,left) or ABflo® 647 Rabbit anti-Human/Mouse CD55/DAF mAb(A23898,5 μl/Test,right). |
 | Flow cytometry:1×10^6 Hela cells were surface-stained with ABflo® 647 Rabbit IgG isotype control (A22070,5 μl/Test,left) or ABflo® 647 Rabbit anti-Human/Mouse CD55/DAF mAb(A23898,5 μl/Test,right). |
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