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ABflo® 647 Rabbit anti-Mouse VISTA PolymAb® (100 T)
| Reactivity: | Mouse |
| Applications: | FC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal Antibody |
| Conjugation: | ABflo® 647. Ex:648nm. Em:664nm. |
| Gene Name: | V-set immunoregulatory receptor |
| Gene Symbol: | Vsir |
| Synonyms: | Dies1; PD-1H; VISTA; 4632428N05Rik |
| Gene ID: | 74048 |
| UniProt ID: | Q9D659 |
| Clone ID: | 2D5A4 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 33-191 of mouse VISTA (NP_083008.1). |
| Dilution: | FC,5 μl per 10^6 cells in 100 μl volume |
| Purification Method: | Affinity purification |
| Concentration: | 0.5mg/ml |
| Buffer: | PBS with 0.03% proclin300,0.2% BSA, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-Vsir antibody |
Background
Predicted to enable endopeptidase activator activity; enzyme binding activity; and identical protein binding activity. Acts upstream of or within several processes, including negative regulation of CD4-positive, alpha-beta T cell proliferation; negative regulation of T cell cytokine production; and positive regulation of BMP signaling pathway. Located in external side of plasma membrane. Is expressed in several structures, including heart; immune system; lung; male reproductive gland or organ; and small intestine lamina propria. Orthologous to human VSIR (V-set immunoregulatory receptor).
Images
 | Flow cytometry: 1×10^6 NIH/3T3 cells (negative control,left) and C57BL/6 mouse splenocytes (right) were surface-stained with ABflo® 647 Rabbit anti-Mouse VISTA PolymAb® (A25974PM,5 μl/Test,orange line) or ABflo® 647 Rabbit IgG isotype control (A22070,5 μl/Test,blue line). Non-fluorescently stained cells were used as blank control (red line). |
 | Flow cytometry: 1×10^6 C57BL/6 mouse splenocytes were surface-stained with ABflo® 647 Rabbit IgG isotype control (A22070,5 μl/Test,left) or ABflo® 647 Rabbit anti-Mouse VISTA PolymAb® (A25974PM,5 μl/Test,right). |
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